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Confirmation of an Enzyme-Linked Immunosorbent Assay to Detect Fluometuron in Soil

Published online by Cambridge University Press:  20 January 2017

Mark W. Shankle ,
David R. Shaw  and
Michele Boyette
Mark W. Shankle
Affiliation:
North Mississippi Research and Extension Center, 8320 Highway 15 South, Pontotoc, MS 38863
David R. Shaw*
Affiliation:
Mississippi State University, Mississippi State, MS 39762
Michele Boyette
Affiliation:
Mississippi State University, Mississippi State, MS 39762
*
Corresponding author's E-mail: [email protected].
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Abstract

Research was conducted to compare the results of an enzyme-linked immunosorbent assay (ELISA) to high-performance liquid chromatography (HPLC) for detecting fluometuron in the environment. A linear relationship for HPLC (R2 > 0.90) and ELISA (R2 > 0.66) analysis was observed between the natural logarithm of the detected fluometuron concentrations regressed against time in soil collected from a cropped area, a grass filter strip, and a riparian forest. Both methods detected the same initial fluometuron concentration (y-intercept) for two of the three soils evaluated. The ELISA and HPLC measurements of fluometuron concentrations compared favorably with r values from 0.83 to 0.98. Predicted fluometuron half-lives determined from HPLC and ELISA measurements were: 110 and 112 d in the cropped watershed, 28 and 29 d in the riparian area, and 11 and 11 d in the grass filter strip, respectively. Results from both techniques indicated shorter half-lives in soil from the grass filter strip and riparian area than in cropped area soil. There was an inverse correlation between predicted half-lives and soil organic matter, pH, clay, and cation exchange capacity.

Keywords

Herbicide degradationBMPs, best management practicesCEC, cation-exchange capacityDAT, days after treatmentDT50, 50% disappearance timeELISA, enzyme-linked immunosorbent assayHPLC, high-performance liquid chromatographyIC50, analyte concentration that decreases chromogen activity by 50%LDD, least detectable doseMD-MSEA, Mississippi Delta Management Systems Evaluation AreaOM, organic matter
Type
Research
Information
Weed Technology , Volume 15 , Issue 4 , December 2001 , pp. 669 - 675
Copyright
Copyright © Weed Science Society of America 

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