An important factor in controlling invasive plant infestations is frequently the acceleration of the deterioration of their persistent seed bank, which is often associated with physical dormancy mechanisms. We hypothesized that breaking dormancy by heat would enhance the vulnerability of the nondormant seeds to hydrothermal stresses. The aim of the present study was to examine the effect of soil solarization treatments (heating the soil by means of polyethylene mulching) on buried Australian Acacia seeds, with emphasis on Acacia saligna L. The results of three field experiments indicate that soil solarization treatments caused an almost complete eradication of buried seeds of Acacia saligna and two other Australian Acacia species, Acacia murrayana and Acacia sclerosperma. The killing mechanism of solarization was further studied in laboratory experiments. We observed two phases of the heat-induced deterioration of seed persistence: breaking the dormancy of the seeds and exposing the “weakened seeds” to lethal temperatures. From an ecological perspective of conservation, the present study shows for the first time the possible utilization of solar energy, by means of soil solarization, for reducing persistent seed banks of invasive woody plants.

Recent studies have shown major advantages of increased crop density and spatial uniformity for competition of wheat with weeds. Field experiments were performed over 3 yr to determine whether the effects of crop density and sowing pattern on weed suppression are influenced by nitrogen fertilization. The independent variables were crop sowing pattern (normal rows and a highly uniform pattern), seeding density (204, 449, and 721 seed m−2) and nitrogen fertilization (0 and 80 kg nitrogen ha−1) of spring wheat, grown under high weed pressure. Increased crop density had strong and consistent negative effects on weed biomass and positive effects on crop biomass and yield. At the highest crop density, weed biomass was less than half that at the lowest density. Weed biomass was generally lower, and yield higher, in the uniform pattern, except in one case in which a combination of factors gave one weed species an early size advantage over the crop. When weeds were controlled with herbicide, no effects of crop density or spatial uniformity on crop biomass or yield were observed. Nitrogen fertilization increased weed biomass in 2 of 3 yr, and it also increased crop biomass in 2 of 3 yr, but there was little evidence that the relative effects of crop density and spatial pattern on weed suppression were influenced by nitrogen fertilization. In the presence of weeds, the highest yields were obtained with high crop density, high spatial uniformity and nitrogen fertilization. The results indicate that increased weed suppression through increased crop density and spatial uniformity will occur over a wide range of nitrogen levels.

Siam weed and coat buttons are among the most troublesome Asteraceae weed species of rainfed rice. The influence of various environmental factors on seed germination and seedling emergence of these weeds was determined. Germination response of both species was greater at the warmer fluctuating temperatures (30/20 and 35/25 C) than at the colder temperatures (25/15 C). Light stimulated germination in both species; however, some seeds still germinated in the dark. Both species were moderately tolerant of salt and water stress, but Siam weed tolerated more stresses than coat buttons. At the soil surface, Siam weed and coat buttons emergence was 75 and 76%, respectively, but this declined rapidly with increasing soil depths. Neither species emerged from depths exceeding 3 cm. Seedling emergence and seedling dry matter of both species were greatly reduced with the addition of crop residue to the soil surface at rates equivalent to 4 to 6 t ha−1. The information gained in this study will be used to facilitate development of effective weed control programs.

The variability of 12 Echinochloa populations with respect to certain morphological and physiological characteristics, sensitivity to certain POST rice herbicides, and activity of selected enzymes was studied. Three distinct groups (each one represented by four populations from different areas) were studied: E. crus-galli, E. oryzoides, and E. phyllopogon. All the E. oryzoides and E. phyllopogon populations showed earlier seed germination and higher germination percentages than the E. crus-galli populations. All the E. oryzoides and E. phyllopogon populations showed reduced susceptibility to propanil, cyhalofop, clefoxydim, and bispyribac compared with the E. crus-galli populations. With respect to plant prostrateness, the species order, averaged over the four populations, was E. crus-galli prostrate > E. oryzoides relatively erect > Ε. phyllopogon phyllopogon. erect, while the species order with respect to leaf length and tillering ability was E. crus-galli ≥ E. oryzoides > Ε. phyllopogon. Regarding leaf width, time of panicle emergence, height, and biomass accumulation, the order was E. crus-galli > E. oryzoides > Ε. phyllopogon, while that of seed weight, length and width was E. oryzoides > Ε. phyllopogon > E. crus-galli. The order of species susceptibility (averaged over the four populations) to most of the herbicide treatments was E. crus-galli > E. oryzoides > E. phyllopogon, which was exactly the opposite of that relating to their antioxidant enzyme activity. Finally, the order of herbicide efficacy, averaged over all Echinochloa populations, was penoxsulam > clefoxydim > bispyribac > cyhalofop > propanil. Variability in a number of traits among the most common Echinochloa species of rice fields in northern Greece as a result of different adaptive strategies of each species may be related to differential sensitivity to herbicides. This variability should be taken into account for the elaboration of effective weed management programs in rice. Where mixed populations of these species are present in a field, difficulties may arise in the successful chemical control of the Echinochloa complex in rice due to species differences in biology and herbicide sensitivity.

Certain winter annual weeds have been documented as alternative hosts to soybean cyst nematode (SCN), and infestations of such species have become common in no-till production fields in the Midwest. This research was conducted to determine the influence of herbicide- and cover-crop-based winter annual weed management systems and crop rotation on winter annual weed growth and seed production, SCN population density, and crop yield. Two crop rotations (continuous soybean and soybean-corn) and six winter annual weed management systems (a nontreated control, fall and spring herbicide applications, spring-applied herbicide, fall-applied herbicide, fall-seeded annual ryegrass, and fall-seeded winter wheat) were evaluated in no-tillage systems from fall 2003 to 2006 at West Lafayette, IN and Vincennes, IN. Fall or spring herbicide treatments generally resulted in lower winter annual weed densities than cover crops. Densities of henbit and purple deadnettle increased over years in the cover crop systems but remained constant in the herbicide systems. Averaged over sites and years, winter annual weed densities were nearly 45% lower in the spring than the fall due to winter mortality. Corn yield was reduced by the cover crops at West Lafayette but not Vincennes. Winter annual weed management system had no influence on soybean yield. SCN population density was reduced by including corn in the crop sequence but was not influenced by winter annual weed management. The density of weedy host species of SCN in the experimental area was relatively low (less than 75 plants m−2) compared to densities that can be observed in production fields. The results of these experiments suggest that inclusion of corn into a cropping sequence is a much more valuable SCN management tool than winter annual weed management. In addition, control of winter annual weeds, specifically for SCN management, may not be warranted in fields with low weed density.

The main objective of the Emerging Technologies Symposium at the 2007 WSSA Annual Meeting was to provide the weed science community with the principles behind emerging technologies and how they can be used to study weed biology. Specifically, aspects and applications related to genomic database development, microarrays, bioinformatics, real-time polymerase chain reaction (PCR), marker development, and proteomics were presented. These technologies have already been used to make significant advances to our understanding of animal and plant biology and are expected to be equally beneficial within the weed science community.

The effects of nitrogen (N) rate and weed interference on the grain yield of four corn genotypes were investigated in 2002 and 2003 at Ikenne (7°38′N, 3°42′E), Shika (11°11′N, 7°38′E), and Samaru (10°24′N, 7°42′E) in Nigeria. Nitrogen (N) at 0, 30, 60, and 90 kg N ha−1 were the main plot treatments. Weed-free (weeded weekly), low (intrarow weeds only), and high (zero weeding) weed pressure were the subplot treatments. Four corn genotypes (ACR8328 BN C7, Low-N-Pool C2, Oba Super II, TZB-SR) were the sub-subplot treatments. Weed density was higher at Shika and Samaru than at Ikenne, and the order of average weed biomass 8 to 10 weeks after planting was Samaru (271 g m−2) > Ikenne (236 g m−2) > Shika (161 g m−2). Corn genotype and N rate had no effect on weed biomass except at Samaru where fertilized treatments had higher weed biomass than the unfertilized treatments. Corn leaf area (LA) increased with increasing N rate at all locations regardless of weed pressure and genotype, except at Shika where ACR8328 BN C7, Oba Super II, and TZB-SR did not show any clear N response; LA was highest in the weed-free and lowest in the unfertilized treatments for all genotypes and locations, and weed pressure treatments. Low-N-Pool C2 had the highest LA, which was 1.3 times larger than in Oba Super II, which had the lowest LA. Nitrogen rate, weed pressure, and genotypes significantly affected corn leaf chlorophyll content. Chlorophyll content was higher in the fertilized treatments than the unfertilized treatments, and higher in the weed-free treatments than the low or high weed pressure treatments. ACR8328 BN C7 and Oba Super II had significantly more chlorophyll than the other genotypes. Low-N-Pool C2 showed a linear grain yield response with the increase in N rates. ACR8328 BN C7 did not respond to N application. Compared with the results in the weed-free treatment, high weed pressure reduced grain yield in all genotypes by more than 65% at Samaru, 50% at Shika, and 35% at Ikenne.

The influence of the holoparasite branched broomrape on the vegetative growth, leaf chlorophyll content, photosynthetic rate, and chlorophyll fluorescence of tomato was studied over two growing seasons on plants grown in a commercial greenhouse. The presence of the parasite strongly reduced the aerial biomass by acting as a competing sink for assimilate, but more importantly, by compromising the efficiency of carbon assimilation via a reduction in leaf chlorophyll content and photosynthetic rate. The chlorophyll fluorescence parameters F0, Fm, Fv, and Fv/Fm were all altered in parasitized plants, indicating that branched broomrape–infected plants are more susceptible to photoinhibition. The degree of damage to the host was not dependent on either the number or the biomass of parasitic plants per host plant. We suggest that the ability to maintain a high photosynthetic rate, leaf chlorophyll content, or both and the ability to minimize photoinhibition can be developed as indirect assays for improved tolerance to branched broomrape.

The rapid expansion of the plant bioeconomy is creating strong economic incentives to distribute novel plant material, including transgenic cultivars, exotic species, and species that were formerly constrained to small geographical areas, at large geographical scales. Such introductions carry with them the risk of invasive spread of the introduced species (Simberloff and Alexander 1998). Deployment of plant species for biofuel production offers a clear example of the benefits and risks associated with the new bioeconomy (Raghu et al. 2006).

In a measure aimed at reducing U.S. dependence upon foreign petroleum reserves for energy production, President Bush announced the Advanced Energy Initiative (AEI) in his 2006 State of the Union address. This initiative provides federal funding and guidelines for the development of renewable energy sources, including plant biofuels. The objectives of the AEI, though admirable, have the potential to create a conflict with Executive Order 13112, which states that “[Federal agencies shall] not authorize, fund, or carry out actions that it believes are likely to cause or promote the introduction or spread of invasive species in the United States or elsewhere unless, pursuant to guidelines that it has prescribed, the agency has determined and made public its determination that the benefits of such actions clearly outweigh the potential harm caused by invasive species; and that all feasible and prudent measures to minimize risk of harm will be taken in conjunction with the actions.”

Additive experiments were performed to determine optimum densities for nematode-suppressive cover crops to extend the benefit from the cover crops by also using them for weed suppression. In a preliminary experiment in 2002, a range of cover-crop densities was evaluated in mixtures with smooth pigweed at 5 plants m−2. Smooth pigweed biomass accumulation was suppressed by cowpea, sunn hemp, and velvetbean at the lowest cover-crop populations (38, 44, and 15 plants m−2, respectively). Based on these results, experiments were conducted in 2003 at two locations to examine the effects of lower cover-crop densities on a higher smooth pigweed population density of 15 plants m−2. Cowpea and velvetbean densities ranged from 10 to 50 plants m−2 and sunn hemp from 20 to 100 plants m−2. In 2003, cowpea density had no effect on smooth pigweed biomass. However, smooth pigweed biomass declined linearly by 51% as sunn hemp density increased to 100 plants m−2. Similarly, as velvetbean densities increased, smooth pigweed biomass decreased showing a linear response at one location and quadratic response at the second location. Maximum suppression of smooth pigweed biomass by velvetbean occurred at the highest cover-crop density (50 plants m−2). Excellent suppression of smooth pigweed at 5 plants m−2 or fewer will result in densities of 38, 44, and 15 plants m−2 of cowpea, sunn hemp, and velvetbean. However, with smooth pigweed at 15 plants m−2, optimum cover-crop densities were not obtained because no suppression was obtained with cowpea, and the lowest weed biomass with sunn hemp and velvetbean occurred with the highest densities used. Therefore, when high smooth pigweed densities are expected, sunn hemp and velvetbean should be used at densities greater than 100 and 50 plants m−2, respectively, and further study with higher densities will be needed to define optima.

Field and greenhouse experiments were conducted to determine the level of glyphosate resistance in common waterhemp populations from Platte County (MO1) and Holt County, Missouri (MO2), and to determine the level and distribution of resistance to glyphosate, acetolactate synthase (ALS)–inhibiting herbicides, and protoporophyrinogen oxidase (PPO)–inhibiting herbicides across the MO1 site. Results from greenhouse experiments revealed that the MO1 and MO2 waterhemp populations were 19 and 9 times more resistant to glyphosate, respectively, than a susceptible waterhemp population. In field experiments, greater than 54% of waterhemp at the MO1 site survived 1.7 kg glyphosate ae ha−1 (twice the labeled rate) 6 wk after treatment. Tank-mix combinations of ALS- and PPO-inhibiting herbicides with glyphosate also failed to provide complete control of the waterhemp population at the MO1 site. Collection and screening of seed from individual female waterhemp accessions revealed multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides across the MO1 site. All 14 waterhemp accessions collected across the MO1 site exhibited greater than 65% survival to 2× rates of glyphosate and thifensulfuron, and these accessions were spread across a 5-km2 (503-ha) area. Four waterhemp accessions collected across a 0.9-km2 (87-ha) area also exhibited 26 to 38% survival to 2× rates of lactofen. The results from these experiments provide evidence and confirmation of the first glyphosate-resistant waterhemp population in the United States and reveal that multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides can occur in waterhemp.

Modern invasion biology is a new science, with the holy grail of being able to predict the trajectory of particular invasions. Although this goal has yet to be achieved, there has been much progress through experimental research and meticulous study of the scope and mechanisms of existing invasions. Several well-established patterns are relevant to potential biofuel feedstocks: (1) ca. half of all damaging plant invaders were deliberately introduced, not accidental hitchhikers or escapees; (2) some native plants have become invasive; coevolution with native community members was not proof against unexpected damage; (3) many introduced plants were innocuous for decades or even centuries in their new locations before suddenly exploding across the landscape; lack of current observed impact does not guarantee safety; and (4) control or even eradication of widespread invaders is sometimes possible, but it is far from certain and it is often very expensive. We cannot count on effectively managing an introduction gone awry. Because much invasion biology is targeted at developing methods of preventing anthropogenic movement or establishment of species, invasion biologists have occasionally been assailed as obstructionists by various interests who fear their livelihoods will be impeded: the seed and horticulture trades, foresters, the pet industry, fish and game biologists, etc. A fringe group of philosophers, sociologists, landscape architects, and others have even taken to calling invasion biology a thinly veiled form of xenophobia. Some biofuels advocates have joined this litany, accusing invasion biologists of playing on the emotions of an uneducated public by raising fears of a new kudzu. Invasion biologists need not be cast in this role. In collaboration with agronomists, geneticists, physiologists, and other scientists, they have much to offer in understanding the risks posed by particular feedstocks and developing approaches that would minimize these risks and mitigate unforeseen consequences.

Quinoclamine is an herbicide under development for control of liverwort, a weed common in nursery crops. With respect to liverwort control, quinoclamine has been considered to primarily have POST activity. However, some PRE activity has been reported. Growth media sorption studies with 14C-quinoclamine indicate that only 0.64% of the quinoclamine amount that enters the media remains unadsorbed and thus available to be taken up by established plants or propagules. Computer modeling revealed that a large portion of the surface of the quinoclamine molecule is positively charged, which likely is the reason for its high adsorptivity. In a simulation of PRE activity, hydroponically grown liverwort and germinating gemmae were exposed to increasing quinoclamine concentrations. Phytotoxicity to both plants and gemmae was obtained with a minimal concentration of 4 to 6 mg L−1. Based upon the projected use rate, and assuming minimal vertical infiltration depth, the theoretical concentration of quinoclamine within the aqueous phase of a pine bark substrate would be approximately 8 mg L−1. In toto, results indicate that the projected use rate will result in sufficient quinoclamine in the aqueous phase of a pine bark substrate to provide PRE control of gemmae propagules as well as to contribute to the efficacy of POST applications to established liverwort.

Defoliation can affect plant competition in at least two ways: negative effects on the performance of defoliated plants and positive effects on nondefoliated plants due to neighbor defoliation. It has been suggested that these effects can alter competitive relations between plants, and this study was designed to test this in an experiment using Crofton weed as the invasive category, and its two neighbors Japanese brome and Dichrocephala as the native category. The plants were grown from seed and potted either singly or in pairs. The pairs consisted either of two conspecific plants (same pairs) or of one plant of each category (mixed pairs). Randomly preselected plants were defoliated twice in the growing season, at 9 wk and again at 5 wk before final biomass sampling. Competition reduced growth by 51 to 78%, with the plants of the native category consistently more affected than the invasive category. When grown singly, Crofton weed grew larger (average 67%) than the native species. As compared with plants grown singly, Crofton weed plants grown in mixed pairs were 40% smaller. The corresponding data for the native species were 78% smaller for Japanese brome and 73% smaller for Dichrocephala. The effects of neighbor defoliation differed among categories and competition. All the nondefoliated plants grown singly were larger than plants grown with a defoliated conspecific neighbor. For the corresponding relationship in mixed pairs, the native plants grown singly were significantly larger than the same native species grown together with a defoliated, invasive neighbor. Defoliation of Crofton weed reduced the growth of native neighbors and increased negative competitive effects on native plants. For the invasive species, however, Crofton weed plants grown in mixed pairs with neighbor defoliation compensated fully for the competitive effects, i.e., they were not significantly different from nondefoliated plants grown singly. Consequently, the effects of defoliation on competition between invasive Crofton weed and its native neighbors may depend on the ability to undertake compensatory growth, and probably on the allelopathic exudation of Crofton weed to herbivory. Our data suggest that herbivory by biocontrols can make an already superior competitor even stronger, especially if the biocontrol agent does not effectively damage or kill the target plant. Owing to complex interactions between competition and defoliation, the indirect effects of herbivory may be more complicated than currently conceived, and understanding the indirect effects of biocontrol agents before their release is crucial.

Three major hypotheses were examined in this study: (1) the density of summer annual weeds is reduced in crop rotation systems that include winter wheat compared to those with strictly summer annual crops, (2) the integration of a red clover in cropping systems reduces weed seedbank densities, and (3) changes in weed seedbanks due to crop rotation system have greater impact on future crops that are managed with cultivation alone, compared to those managed with herbicides. To test these hypotheses, five 3-yr rotation sequences were examined in central New York state: continuous field corn (FC); field corn with red clover (FC + CL); field corn–oats–wheat (FC/O/W); sweet corn–peas–wheat (SC/P/W), and SC/P/W with red clover (SC/P/W + CL). In the fourth year, sweet corn, snap beans, and cabbage were planted in subplots with three levels of weed management as sub-subplots: cultivation alone, reduced-rate herbicides (1/2×), and full-rate herbicides (1×). The trial was carried out in two separate cycles, from 1997 to 2000 (cycle 1) and from 1998 to 2001 (cycle 2). Crop rotations with strictly summer annual crops (FC) did not result in consistently higher weed seedbank densities of summer annual weeds compared to rotations involving winter wheat (FC/O/W; SC/P/W; SC/P/W + CL). Integration of red clover in continuous field corn resulted in higher weed seedbanks (cycle 1) or emergence (cycle 2) of several summer annual weeds compared to field corn alone. In contrast, integration of red clover in the SC/P/W rotation led to a 96% reduction in seedbank density of winter annuals in cycle 1, although this effect was not detected in cycle 2. Observed changes in weed seedbank density and emergence due to crop rotation resulted in increased weed biomass in the final year in only one case (sweet corn, cycle 2), and did not result in detectable differences in crop yields. In contrast, final year weed management had a strong effect on weed biomass and yield; cultivation alone resulted in yield losses for sweet corn (32 to 34%) and cabbage (0 to 7%), but not snap beans compared to either 1/2× or 1× herbicides.

DNA microarrays are one of the new tools of genomics that have become quite commonplace in plant science research within the past decade. Essentially, DNA microarrays are an extension of the more traditional molecular biology technique of northern blotting. Unlike northern blotting, however, in which the expression of a single gene is monitored, DNA microarrays allow for the simultaneous monitoring of thousands of genes—or, in fact, potentially all of an organism's genes—in a single experiment. Most of the currently available plant microarrays are designed for crops species or the model plant, Arabidopsis; however, a microarray for at least one major weed species is currently available. Furthermore, cDNA-based microarrays prepared for one species can be used to investigate gene expression in related species. As the technology is maturing it is becoming much more accessible, and now is the time to begin utilizing microarrays in weed science research. Questions related to herbicide activity are particularly well suited for a microarray approach. Additionally, gene-expression profiling via microarrays can be used to address questions relating to weed biology, weed–crop competition, allelopathy, parasitic weeds, and biological control of weeds.

This study investigated factors that influence occurrence and persistence of plant DNA in the soil environment in three crop rotations. In each rotation, soil was sampled in May before planting, in July and August while crops were growing, and in October after harvest. Total DNA was recovered from soil samples taken at two different depths in the soil profile and quantified. Three target plant genes (corn CP4 epsps, corn 10-kD Zein, and soybean CP4 epsps) also were quantified in these DNA extracts using species-specific quantitative real-time PCR assays. In general, total plant DNA content in the soil environment was greatest when the crop was growing in the field and decreased rapidly after harvest. Nevertheless, low levels of target plant DNA were often still detectable the following spring. Age of rotation did not influence target DNA quantities found in the soil environment. Data were collected for a combination of 10 location-years, which allowed for estimation of the variance components for six factors including time of sampling, year, location, crop, sampling depth, and herbicide to total and target DNA content in the soil samples. Mean target recombinant DNA content in soil was influenced most strongly by time of sampling and year (85 and 6%, respectively), whereas total soil DNA content was less dynamic and was most strongly influenced by location and year (49 and 25%, respectively). Over the duration of this study, no accumulation of transgenic plant DNA in the soil environment was observed.

Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is used to determine relative gene expression or to quantify exact levels of mRNA in cells or tissues. Before the advent of real-time PCR, the major difficulty associated with traditional quantitative or semiquantitative PCR was to ensure that PCR reactions were quantified within the exponential phase of amplification. Real-time PCR alleviates that problem by detecting and quantifying fluorescent signals after each amplification cycle. Additionally, it does not require running gels and thus is able to produce data in 2 to 3 h. Four different types of chemistries, DNA-binding agents (SYBR Green), hydrolysis probes (TaqMan), hairpin probes (molecular beacons, scorpions), and fluorescent-labeled hybridization probes (Light Cycler), have been commonly used for real-time PCR. Among those chemistries, SYBR Green is the most economical choice. We have used real-time PCR and SYBR Green to examine the expression of a number of leafy spurge genes after growth induction and during normal seasonal growth. Because no reliable endogenous reference genes have been identified in leafy spurge, we performed PCR without an endogenous reference gene and analyzed messenger RNA (mRNA) expression based on the threshold cycle (CT) value of amplification. Excluding an endogenous reference gene from that data analysis was rather straightforward and reliable if RNA was properly prepared and quantified. Given that genomic tools, such as expressed sequence tags (ESTs), and their expression profiles are lacking for most weedy species, avoiding the use of endogenous reference genes in real-time PCR simplifies the optimization process and reduces the cost tremendously. However, we found that using a passive reference dye (ROX) to normalize non-PCR–related fluctuations in fluorescent signal is desirable.

Cover crop systems were investigated in 2004 and 2005 for their effects on the activity-density (a function of movement and density) of a promising group of weed biocontrol organisms, the ground beetles collectively known as carabids, with particular emphasis on a beneficial carabid species Harpalus rufipes DeGeer. Marked H. rufipes released into pea/oat–rye/vetch cover crop plots were more than twice as likely to be recaptured within the same plots as beetles released in nonvegetated fallow plots (18 and 8%, respectively). Marked beetles released into fallow plots were more than twice as likely to leave their plots and be recaptured in pea/oat–rye/vetch plots as vice versa (13 vs. 5%), indicating a clear preference for habitat with vegetative cover. Overall recapture rates were not different between treatments. Unmarked H. rufipes activity-density was also higher in pea/oat–rye/vetch compared to fallow plots. Additionally, five cover crop systems, including the fallow and pea/oat–rye/vetch treatments, and two residue management methods (conventional and zone tillage) were investigated from June to August in 2005 for their effects on H. rufipes activity-density. Corn was planted in 2005 into residues of the five cover crop systems grown in 2004. H. rufipes activity-density was higher in zone and conventionally tilled corn planted in pea/oat–rye/vetch residues and conventionally tilled corn planted in red clover/oat residues than in any other cover crop and residue management combination. Pea/oat–rye/vetch cover crop systems are apparently beneficial for H. rufipes during the cover crop year as well as in subsequent crops planted into this cover crop's residues. This system was not the least disturbed system but, based on the number of tillage events, represented a medium level of disturbance among the various systems. Thus, some level of disturbance might be beneficial for H. rufipes, but how and when that soil disturbance occurs requires further research to determine the best means of conserving this species.

Dodder is a parasitic weed that is troublesome to the growth of many plants. Our study shows that this invasive species contains strong allelopathic potential, exerting strong inhibition against the growth of indicator plants and noxious paddy weeds in bioassay and pot trials. In a greenhouse, incorporation of 0.5 t ha−1 of dried dodder plants to paddy soil reduced spontaneous growth of paddy weeds by about 50%, whereas the 1.5 to 2 t ha−1 dose suppressed biomass of paddy weeds by more than 75% and completely controlled emergence of barnyardgrass and monochoria. By the use of a separation resin, 22 compounds were separated from dodder and identified by gas chromatography–mass spectrometry as belonging to terpenes, long-chain fatty acids, phenols, phenolic acids, and lactone. Among these compounds, 15 substances were quantified and tested for their herbicidal activity. Quantity of cinnamic acid was the highest (37.3 mg g−1), followed by dihydro-5,6-dehydrokavain (DDK; 6.0 mg g−1), myristic acid (3.2 mg g−1), and methyl cinnamate (2.1 mg g−1), whereas the amounts of other compounds were between 0.01 and 0.1 mg g−1. It is suggested that the content of the terpenes within dodder, which was rather high in amount (0.41–2.1 mg g−1), correlated to its strength of chemical cues to find host plants. Cinnamic acid, DDK, methyl cinnamate, and vanillin exerted the most potent herbicidal activities against radish growth. Findings of this study propose that cinnamic acid, DDK, and methyl cinnamate are responsible for its strong phytotoxic action of dodder plants. However, whether these plant growth inhibitors and other compounds detected from the dodder can suppress emergence of their hosts as well as contributing to its strong invasiveness needs further elucidation.