Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-18T20:03:13.687Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunocytochemical reactivity of rod and cone visual pigments in the sturgeon retina

Published online by Cambridge University Press:  02 June 2009

Victor I. Govardovskii ,
Pál Röhlich ,
Ágoston Szél  and
Lida V. Zueva
Victor I. Govardovskii
Affiliation:
Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Petersburg, Russia
Pál Röhlich
Affiliation:
Laboratory 1 of Electron Microscopy, Semmelweis University of Medicine, Budapest, Hungary
Ágoston Szél
Affiliation:
Laboratory 1 of Electron Microscopy, Semmelweis University of Medicine, Budapest, Hungary
Lida V. Zueva
Affiliation:
Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Petersburg, Russia
Get access Rights & Permissions [Opens in a new window]

Abstract

Microspectrophotometry and immunocytochemistry with several antivisual pigment antibodies were used to study visual cells of the Siberian sturgeon, Acipenser baeri Brandt. The retina contained rods and three morphological types of cones: large cones with oil drops, small cones with oil drops, and cone-like cells without oil drops. Rods and cone-like drop-free cells were found to possess porphyropsin-549, while the large oil drop-bearing cones contained red-sensitive (P613), green-sensitive (P542), and blue-sensitive (P462) visual pigments. The immunocytochemical staining pattern with three antibodies to visual pigment proteins also revealed one visual pigment in rods and three visual pigments in cones. Rods were labeled with all three antibodies, while the majority of large cones (type I), presumably the red-sensitive ones, were negative with the polyclonal serum AO against bovine opsin. A less-frequently occurring large cone type (type II) was stained by all three antibodies including mAb COS-1 specific to middle-to-long-wave visual pigments in birds and mammals, and is thought to be green-sensitive. An even less-frequent large cone type (type III, probably the blue-sensitive one) did not bind COS-1. The small cones with oil droplets showed immunoreactivities similar to either type II or type III cones. The oil drop-free small photoreceptor exhibited a staining pattern identical with that of rods. These results indicate that the immunocytochemical approach can be used to reveal photoreceptor-specific neural connections in the sturgeon retina.

Keywords

SturgeonPhotoreceptorsVisual pigmentsMonoclonal antibodiesImmunocytochemistry
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 6 , June 1992 , pp. 531 - 537
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Burkhardt, D.A., Gottesman, J., Levine, J.S. & MacnlcholJ.S., Jr. J.S., Jr. (1983). Cellular mechanisms for colorcoding in holostean retina and evolution of colour vision. Vision Research 23, 1031–1041.CrossRefGoogle Scholar
Crescitelli, F. (1972). The visual cells and visual pigments of the vertebrate eye. In Handbook of Sensory Physiology, Vol. VII/I, ed. Dartnall, H.J.A., pp. 333–337. Berlin: Springer Verlag.Google Scholar
CserhÁti, P., Szél, Á., & Röhlich, P. (1989). Four cone types characterized by antivisual pigment antibodies in the pigeon retina. Investigative Ophthalmology and Visual Science 30, 74–81.Google ScholarPubMed
Dawis, S.M. (1981). Polynomial expression of pigment nomograms. Vision Research 21, 1427–1430.CrossRefGoogle ScholarPubMed
Govardovskii, V.I., Byzov, A.L., Zueva, L.V., Policzuk, N.A. & Baburina, E.A. (1991). Spectral characteristics of photoreceptors and horizontal cells in the retina of the Siberian sturgeon, Acipenser baeri Brandt. Vision Research 31, 2047–2056.CrossRefGoogle ScholarPubMed
Govardovskii, V.I. & Zueva, L.V. (1987). Photoreceptors and visual pigments in sturgeons. Journal of Evolutionary Biochemistry and Physiology (Russia) 23, 685–686.Google Scholar
Govardovskii, V.I. & Zueva, L.V. (1988). A simple, highly-sensitive recording microspectrophotometer. Cytology (Russia) 30, 499–502.Google ScholarPubMed
Johns, P.R. & Fernald, R.D. (1981). Genesis of rods in teleost fish retina. Nature 293, 141–142.CrossRefGoogle ScholarPubMed
Levine, J.S. & MacnicholE.F., Jr. E.F., Jr. (1985). Microspectrometry of primate photoreceptors: artifact and analysis. In The Visual System, ed. Fein, A. & Levine, J.S., pp. 73–88, New York: Liss.Google Scholar
MacnicholE.F., Jr. E.F., Jr. (1986). A unifying presentation of photopigment spectra. Vision Research 26, 1543–1556.CrossRefGoogle ScholarPubMed
Maximov, V.V. (1988). Approximation of visual pigment absorbance spectra. Sensory Systems (Russia) 2, 3–9.Google Scholar
Mayor, H.D., Hampton, J.C. & Rosario, B. (1961). A simple method of removing the resin from epoxy-embedded tissue. Journal of Biophysical and Biochemical Cytology 2, 909–910.CrossRefGoogle Scholar
RÖHlich, P., Szél, Á., & Papermaster, D.S. (1989). Immunocytochemical reactivity of Xenopus laevis retinal rods and cones with several monoclonal antibodies to visual pigments. Journal of Comparative Neurology 290, 105–117.CrossRefGoogle ScholarPubMed
Sillman, A.J., Spanfelner, M.D. & Loew, E.R. (1990). The photoreceptors and visual pigments in the retina of the white sturgeon, Acipenser transmontanus. Canadian Journal of Zoology 68, 1544–1551.CrossRefGoogle Scholar
Stell, W.K., Karkhanis, A. & Bremner, L. (1988). Rod neuroblast proliferation in goldfish: Circadian rhythm and detection. Investigative Ophthalmology and Visual Science 29, 348.Google Scholar
Szél, Á., Diamantstein, T. & Röhlich, P. (1988). Identification of the bluesensitive cones in the mammalian retina by anti-visual pigment antibody. Journal of Comparative Neurology 273, 593–602.CrossRefGoogle ScholarPubMed
Szél, Á., Röhlich, P. & Govardovskii, V. (1986a). Immunocytochemical discrimination of visual pigments in the retinal photoreceptors of the nocturnal gecko Teratoscincus scincus. Experimental Eye Research 43, 895–904.CrossRefGoogle ScholarPubMed
Szél, Á. & Röhlich, P. (1988a). Four photoreceptor types in the ground squirrel retina as evidenced by immunocytochemistry. Vision Research 28, 1297–1302.CrossRefGoogle ScholarPubMed
Szél, Á., & Röhlich, P. (1988b). The mosaic of colour-specific photoreceptors in the mammalian retina as defined by immunocytochemistry. Acta Morphologica Hungarica 36, 191–202.Google ScholarPubMed
Szél, Á., & Röhlich, P. (1989). Colour vision and immunologically identifiable photoreceptor subtypes. In Neurobiology of Sensory Systems, ed. Singh, R.N. & Strausfeld, N.J., pp. 275–293. New York: Plenum Press.CrossRefGoogle Scholar
Szél, Á., Takács, L., Monostori, É., Diamantstein, T., Vigh-Teichmann, I. & Röhlich, P. (1986b). Monoclonal antibody recognizing cone visual pigment. Experimental Eye Research 43, 871–883.CrossRefGoogle ScholarPubMed
Szél, Á., Takács, L., Monostori, É., Vigh-Teichmann, I. & Röhlich, P. (1985). Heterogeneity of chicken photoreceptors as defined by hybridoma supernatants. An immunocytochemical study. Cell and Tissue Research 240, 735–741.CrossRefGoogle ScholarPubMed