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The requirement for DNA repair in desiccation tolerance of germinating embryos

Published online by Cambridge University Press:  19 September 2008

Ivan Boubriak ,
Haroula Kargiolaki ,
Linden Lyne  and
Daphne J. Osborne
Ivan Boubriak
Affiliation:
Oxford Research Unit, Open University, Foxcombe Hall, Boars Hill, Oxford, OX1 5HR, UK
Haroula Kargiolaki
Affiliation:
Oxford Research Unit, Open University, Foxcombe Hall, Boars Hill, Oxford, OX1 5HR, UK
Linden Lyne
Affiliation:
Oxford Research Unit, Open University, Foxcombe Hall, Boars Hill, Oxford, OX1 5HR, UK
Daphne J. Osborne*
Affiliation:
Oxford Research Unit, Open University, Foxcombe Hall, Boars Hill, Oxford, OX1 5HR, UK
*
*Correspondence
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Abstract

It is proposed that desiccation tolerance in the embryo of seeds depends upon the capacity to repair damage to genomic DNA when the desiccated embryo is rehydrated. From a study of imbibed and hydrated embryos of rye (Secale cereale) and wild oat (Avena fatua) evidence is provided that it is neither the extent of water uptake by the cells, the ensuing stability of the DNA to desiccation, nor the onset of S-phase DNA synthesis in the first cell cycle of germination that determines whether the desiccated embryo will survive. It is shown that when α- and β-polymerases of DNA repair are inhibited by aphidicolin and dideoxythymidine-5'-triphosphate, respectively, a γ-irradiation-induced DNA fragmentation cannot be fully repaired. It is shown that in hydrated embryos, at a stage when desiccation tolerance is lost, embryo cells still repair irradiation-induced damage, but this repaired DNA is unstable to desiccation and cannot be rerepaired when water is again made available. The failure to re-repair on rehydration appears to be critical to embryo survival and successful germination.

Keywords

desiccation toleranceembryo survivalDNA integrityDNA repairSecale cerealeAvena fatua.
Type
Biochemistry and Metabolism
Information
Seed Science Research , Volume 7 , Issue 2 , June 1997 , pp. 97 - 106
Copyright
Copyright © Cambridge University Press 1997

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References

Bewley, J.D. (1979) Physiological aspects of desiccation tolerance. Annual Review of Plant Physiology 30, 195–238.CrossRefGoogle Scholar
Bino, R.J., Lanteri, S., Verhoeven, H.A. and Kraak, H.L. (1993) Flow cytometric determinations of nuclear replication stages in seed tissues. Annals of Botany 72, 181–187.CrossRefGoogle Scholar
Bruni, F. and Leopold, A.C. (1991) Glass transition in soybean seed. Relevance to anhydrous biology. Plant Physiology 96, 660–663.CrossRefGoogle ScholarPubMed
Brunori, A. (1967) A relationship between DNA synthesis and water content during ripening of Vicia faba seed. Caryologia 20, 333–338.CrossRefGoogle Scholar
Cheah, K.S.E. and Osborne, D.J. (1978) DNA lesions occur with loss of viability in embryos of ageing rye seeds. Nature (London) 272, 593–599.CrossRefGoogle Scholar
Coello, P. and Vazquez-Ramos, J.M. (1996) Maize DNA polymerase 2 (an α-type enzyme) suffers major damage after seed deterioration. Seed Science Research 6, 1–7.CrossRefGoogle Scholar
Cuming, A.C. and Osborne, D.J. (1978) Membrane turnover in imbibed and dormant embryos of wild oat (Avena fatua L.). 1. Protein turnover and membrane replacement. Planta 139, 208–217.Google Scholar
Elder, R.H. and Osborne, D.J. (1993) Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy. Seed Science Research 3, 43–53.CrossRefGoogle Scholar
Elder, R.H., Dell'Aquila, A., Mezzina, M., Sarasin, A. and Osborne, D.J. (1987) DNA ligase in repair and replication in the embryos of rye, Secale cereale. Mutation Research 181, 61–71.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W., Berjak, P., Farnsworth, E.G. and Vertucci, C.W. (1996) Presence of dehydrinlike proteins and levels of abscisic acid in recalcitrant (desiccation sensitive) seeds may be related to habitat. Seed Science Research 6, 175–182.CrossRefGoogle Scholar
Finch-Savage, W.E. and Clay, H.A. (1994) Water relations of germination in the recalcitrant seeds of Quercus robur L. Seed Science Research 4, 315–322.CrossRefGoogle Scholar
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols — review and survey. Seed Science Research 4, 385–405.CrossRefGoogle Scholar
Illades-Aguiar, B. and Setlow, P. (1994) Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid. Journal of Bacteriology 176, 7032–7037.CrossRefGoogle Scholar
Osborne, D.J. and Boubriak, I.I. (1994) DNA and desiccation tolerance. Seed Science Research 4, 175–185.CrossRefGoogle Scholar
Osborne, D.J. and Cheah, K.S.E. (1982) Hormones and foliar senescence. pp 57–83in Jackson, M.B., Grout, B. and Mackensie, I.A. (Eds) British plant regulator group monograph 8. London, Butterworths.Google Scholar
Sen, S. and Osborne, D.J. (1974) Germination of rye embryos following hydration-dehydration treatments: enhancement of protein and RNA synthesis and earlier induction of DNA replication. Journal of Experimental Botany 25, 1010–1019.CrossRefGoogle Scholar
Setlow, P. (1992) DNA in dormant spores of Bacillus species is in an A-like conformation. Molecular Microbiology 6, 563–567.CrossRefGoogle Scholar
Sun, W.Q, Irving, T.C. and Leopold, A.C. (1994) The role of sugar, vitrification and membrane transition in seed desiccation tolerance. Physiologia Plantarum 19, 621–628.CrossRefGoogle Scholar
van Pijlen, J.G., Groot, S.P.C., Kraak, H.L. and Bergervoet, H.W. (1996) Effects of pre-storage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance in tomato (Lycopersicon esculentum Mill.) seeds. Seed Science Research 6, 57–63.CrossRefGoogle Scholar