Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-29T01:09:10.012Z Has data issue: false hasContentIssue false
  • English
  • Français

Domains on the hepatitis C virus internal ribosome entry site for 40s subunit binding

Published online by Cambridge University Press:  23 August 2002

J. ROBIN LYTLE ,
LILY WU  and
HUGH D. ROBERTSON
J. ROBIN LYTLE
Affiliation:
Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA Present address: Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, Connecticut 06536, USA.
LILY WU
Affiliation:
Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
HUGH D. ROBERTSON
Affiliation:
Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
Get access

Abstract

The internal ribosome entry site (IRES) of the hepatitis C virus (HCV) RNA is known to interact with the 40S ribosomal subunit alone, in the absence of any additional initiation factors or Met-tRNAi. Previous work from this laboratory on the 80S and 48S ribosomal initiation complexes involving the HCV IRES showed that stem-loop III, the pseudoknot domain, and some coding sequence were protected from pancreatic RNase digestion. Stem-loop II is never protected by these complexes. Furthermore, there is no prior evidence reported showing extensive direct binding of stem-loop II to ribosomes or subunits. Using direct analysis of RNase-protected HCV IRES domains bound to 40S ribosomal subunits, we have determined that stem-loops II and III and the pseudoknot of the HCV IRES are involved in this initial binding step. The start AUG codon is only minimally protected. The HCV-40S subunit binary complex thus involves recognition and binding of stem-loop II, revealing its role in the first step of a multistep initiation process that may also involve rearrangement of the bound IRES RNA as it progresses.

Keywords

40S subunithepatitis C virusinternal ribosome entry site (IRES)RNase protectiontranslation initiation
Type
Research Article
Information
RNA , Volume 8 , Issue 8 , August 2002 , pp. 1045 - 1055
Copyright
© 2002 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)