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Comparative Decalcification Methods, Radiocarbon Dates, and Stable Isotopes of the VIRI Bones

Published online by Cambridge University Press:  18 July 2016

Noreen Tuross*
Affiliation:
Department of Human Evolutionary Biology, 11 Divinity Avenue, Harvard University, Cambridge, Massachusetts 02130, USA. Email: [email protected]
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Abstract

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The Fifth International Radiocarbon Comparison (VIRI) provided a suite of 5 bone samples with consensus ages ranging from 969 to 39,305 14C yr BP (Scott et al. 2010). These bones were used herein in a comparison of decalcification methods using either HCl or EDTA to produce collagen, and the results demonstrate age concordance between both preparation methods and the VIRI consensus values. Additional isotopic analyses of the collagen (δ13C, δ15N, and δ18O) illustrate the increasing sensitivity of carbon, nitrogen, and oxygen isotopes in assessing gelatin degradation.

Type
Articles
Copyright
Copyright © 2012 by the Arizona Board of Regents on behalf of the University of Arizona 

References

Arslanov, KA, Svezhentsev, YS. 1993. An improved method for radiocarbon dating fossil bones. Radiocarbon 3 5(3):387–91.Google Scholar
Beaumont, W, Beverly, R, Southon, J, Taylor, RE. 2010. Bone preparation at the KCCAMS laboratory. Nuclear Instruments and Methods in Physics Research B 268(7–8):906–9.Google Scholar
Bensusan, HB, Nielsen, SO. 1964. Deuterium exchange of peptide-group hydrogen atoms during the gelatin to collagen-fold transition. Biochemistry 3(9):1367–72.Google Scholar
Berglund, BE, Hakansson, S, Lagerlund, E. 1976. Radiocarbon-dated mammoth (Mammuthus primigenius Blumenbach) find in South Sweden. Boreas 5:177–91.Google Scholar
Brown, TA, Nelson, DE, Vogel, JS, Southon, JR. 1988. Improved collagen extraction by modified Longin method. Radiocarbon 30(2):171–7.Google Scholar
Capelo, JL, Carreira, RJ, Fernandes, L, Lodeiro, C, Santos, HM, Simal-Gandara, JL. 2010. Latest developments in sample treatment for 18O-isotopic labeling for proteomics mass spectrometry-based approaches: a critical review. Talanta 80(4):1476–86.Google Scholar
Eastoe, JE. 1955. The amino acid composition of mammalian collagen and gelatin. Journal of Biological Chemistry 61(4):5S9600.Google Scholar
El-Daoushy, MFAF, Olsson, IU, Oro, FH. 1978. The EDTA and HCl methods of pre-treating bones. Geologiska Föreningens i Stockholm Förhandlingar 100:213–9.Google Scholar
Flanagan, B, Nichols, G Jr. 1969. Bone matrix turnover and balance in vitro: II. The effects of ageing. Journal of Clinical Investigation 48(4):607–12.Google Scholar
Frank, JD, Balena, R, Masarachia, P, Seedor, G, Cartwright, ME. 1993. The effect of three different demineralization agents on osteopontin localization in adult rat bone using immunohistochemistry. Histochemistry and Cell Biology 99(4):295301.Google Scholar
Gilbert, MTP, Bandelt, H-J, Hofreiter, M, Barnes, I. 2005. Assessing ancient DNA studies. Trends in Ecology and Evolution 20(10):541–4.Google Scholar
Gurfinkel, DM. 1987. Comparative study of the radiocarbon dating of different bone collagen preparations. Radiocarbon 29(1):4552.Google Scholar
Haynes, CV. 1967. Bone organic matter and radiocarbon dating. In: Radiocarbon Dating and Methods of Low-Level Counting. Vienna: International Atomic Energy Agency. p 163–8.Google Scholar
Hedges, REM, van Klinken, GJ. 1992. A review of current approaches in the pretreatment of bone for radiocarbon dating by AMS. Radiocarbon 34(3):279–91.Google Scholar
Higham, TFG, Jacobi, RM, Bronk Ramsey, C. 2006. AMS radiocarbon dating of ancient bone using ultrafiltration. Radiocarbon 48(2):179–95.Google Scholar
Jackson, DS, Bentley, JP. 1960. On the significance of the extractable collagens. Journal of Cell Biology 7(1):3742.Google Scholar
Kirsanow, K, Makarewicz, C, Tuross, N. 2008. Stable oxygen (δ18O) and hydrogen (δD) isotopes in ovicaprid dentinal collagen record seasonal variation. Journal of Archaeological Science 35(12):3159–67.Google Scholar
Liu, H, Fang, HHP. 2002. Extraction of extracellular polymeric substances (EPS) of sludges. Journal of Biotechnology 95:249–56.Google Scholar
Longin, R. 1971. New method of collagen extraction for radiocarbon dating. Nature 230(5291):241–2.Google Scholar
Olsson, I. 2000. Further tests of the EDTA treatment of bones. Radiocarbon 42(1):4952.Google Scholar
Olsson, IU, El-Daoushy, M, Farid, AF, Abd-El-Mageed, AI, Klasson, M. 1974. A comparison of different methods for pretreatment of bones I. Geologiska Föreningens i Stockholm Förhandlingar 96:171–81.Google Scholar
Pestle, WJ. 2010. Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: an exploratory study. Journal of Archaeological Science 37(12):3124–8.Google Scholar
Piszkiewicz, D, Landon, M, Smith, EL. 1970. Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations. Biochemical and Biophysical Research Communications 40(5):1173–8.CrossRefGoogle ScholarPubMed
Salamon, M, Tuross, N, Arensburg, B, Weiner, S. 2005. Relatively well preserved DNA is present in the crystal aggregates of fossil bones. Proceedings of the National Academy of Sciences USA 102(39):13,7838.Google Scholar
Scott, EM, Cook, GT, Naysmith, P. 2010. A report on Phase 2 of the Fifth International Radiocarbon Intercomparison (VIRI). Radiocarbon 52(2–3):846–58.Google Scholar
Sharp, ZD, Atudorei, V, Durakiewicz, T. 2001. A rapid method for determination of hydrogen and oxygen isotope ratios from water and hydrous minerals. Chemical Geology 178(1–4):197210.Google Scholar
Silva, T, Kirkpatrick, A, Brodsky, B, Ramshaw, JAM. 2005. Effect of deamidation on stability for the collagen to gelatin transition. Journal of Agricultural and Food Chemistry 53(20):7802–6.Google Scholar
Tuross, N, Fogel, ML, Hare, PE. 1988. Variability in the preservation of the isotopic composition of collagen from fossil bone. Geochimica et Cosmochimica Acta 54(4):929–35.Google Scholar
Tuross, N, Warinner, C, Kirsanow, K, Kester, C. 2008. Organic oxygen and hydrogen isotopes in a porcine controlled dietary study. Rapid Communications in Mass Spectrometry 22(11):1741–5.Google Scholar
Veis, A. 1964. The Macromolecular Chemistry of Gelatin. New York: Academic Press.Google Scholar
Veis, A, Anesey, J. 1965. Modes of intermolecular crosslinking in mature insoluble collagen. The Journal of Biological Chemistry 240(10):3899–908.Google Scholar
Warinner, C, Tuross, N. 2009. Alkaline cooking and stable isotope tissue-diet spacing in swine: archaeological implications. Journal of Archaeological Science 36(8):1690–7.Google Scholar
Zhang, Z, Guoying, L, Shi, B. 2006. Physiochemical properties of collagen, gelatin and collagen hydrolysate derived from bovine limes split wasters. Journal of the Society of Leather Technologists and Chemists 90:23–8.Google Scholar