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Use of the rumen simulation technique (rusitec) to provide micro-organisms for assessing the rate of fermentation. in vitro, of forages.

Published online by Cambridge University Press:  24 November 2017

J H T Barbi ,
E Owen  and
M K Theodorou
J H T Barbi
Affiliation:
Department of Agriculture, University of Reading, Earley Gate, PO Box 236, Reading, Berks RG2 2AT
E Owen
Affiliation:
Department of Agriculture, University of Reading, Earley Gate, PO Box 236, Reading, Berks RG2 2AT
M K Theodorou
Affiliation:
Institute of Grassland & Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
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Extract

In vitro forage-digestion techniques, such as Tilley & Terry (1963) and the recent Pressure Transducer Technique (PTT) of Theodorou et al (1992), rely on rumen-fistulated animals to provide the microbial inoculum. The present study aimed to minimise reliance on rumen-fistulated animals by using the vessel or effluent fluids from the in vitro rumen simulation technique (RUSITEC) of Czerkawski & Breckenridge (1977) as sources of inocula for assessing rate of fermentation of forages using the PTT. A previous study (Owen et al 1991) demonstrated the feasibility of using RUSITEC effluent as a source of micro-organisms for the Tilley and Terry (1963) technique.

Type
Ruminant Metabolism
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1993: Winter meeting , March 1993 , pp. 172
Copyright
Copyright © The British Society of Animal Production 1993

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References

Czerkawski, J W & Breckenridge, G 1977. Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38: 371–384 CrossRefGoogle ScholarPubMed
McDougall, E I 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochemistry Journal 43: 99–109 CrossRefGoogle ScholarPubMed
Orskov, E R & McDonald, I 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science Cambridge 92: 499–503 CrossRefGoogle Scholar
Owen, E, Jayasuriya, M C N, Hamilton, R & Lalenta, M 1991. Use of a long-term rumen simulatior technique (Rusitec) to provide micro-organisms for the in vitro digestibility assays. Journal of Agricultural Science, Cambridge 116: 297–301 CrossRefGoogle Scholar
Tilley, J M A & Terry, R A 1963. A two-stage technique for in vitro digestion of forage crops. Journal of the British Grassland Society 18: 104–111 CrossRefGoogle Scholar
Theodorou, M K, Williams, B A, Brooks, A, Dhanoa, M S & Gill, M 1992. In Animal Production in Developing Countries Occasional Publication No. 16 (ed Gill, M, Owen, E, Pollot, G E and Lawrence, T L J) British Society of Animal Production.Google Scholar