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Treatment efficacy and regulatory host responses in chronic experimental Schistosoma bovis infections in goats

Published online by Cambridge University Press:  20 April 2006

J. MONRAD ,
K. SÖRÉN ,
M. V. JOHANSEN ,
R. LINDBERG  and
N. ØRNBJERG
J. MONRAD
Affiliation:
Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Dyrlaegevej 100, DK-1870 Frederiksberg C, Denmark
K. SÖRÉN
Affiliation:
Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology and Toxicology, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden
M. V. JOHANSEN
Affiliation:
DBL-Institute for Health Research and Development, Jaegersborg Alle 1D, DK-2920 Charlottenlund, Denmark
R. LINDBERG
Affiliation:
Department of Biomedical Sciences and Veterinary Public Health, Division of Pathology, Pharmacology and Toxicology, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden
N. ØRNBJERG
Affiliation:
DBL-Institute for Health Research and Development, Jaegersborg Alle 1D, DK-2920 Charlottenlund, Denmark
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Abstract

The aim of this study was to elucidate the regulatory responses and the long-term effect of praziquantel treatment in chronically Schistosoma bovis-infected West African Dwarf goats. Forty-two goats were used and the design comprised a primary infection followed by treatment at week 13, challenge infection at week 36 and termination at week 52. Dependent variables included clinico-pathological data, worm numbers, faecal and tissue egg counts, and gross pathology of the liver. The results showed that primary infections remained suppressed for up to 52 weeks and, although challenge infections imposed on 36-week-old primary infections established fully, the impairment of their egg production capacity provided protection against clinico-pathological consequences measured by body weight and haemoglobin levels. The study also confirmed a high efficacy (97·7%) of praziquantel for treatment of S. bovis infection in goats and showed that anthelminthic removal of primary infections does not interfere with the ability of the goat to elicit a marked resistance to a subsequent challenge infection. Although treated goats had more fibrous scarring of livers than untreated goats, no negative effects of liver lesions were reflected in weight gains of treated goats. This study provides strong evidence for the beneficial effects of anthelminthic treatment of young domestic stock as an element of treatment and preventive programmes.

Keywords

Schistosoma bovischronic infectionspraziquantel treatmentresistancegoats
Type
Research Article
Information
Parasitology , Volume 133 , Issue 2 , August 2006 , pp. 151 - 158
Copyright
2006 Cambridge University Press

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References

REFERENCES

Agnew, A., Fulford, A. J. C., Mwanje, M. T., Gachuhi, K., Gutsmann, V., Krijger, F. W., Sturrock, R. F., Vennervald, B. J., Ouma, J. H., Butterworth, A. E. and Deelder, A. M. ( 1996). Age-dependent reduction of schistosome fecundity in Schistosoma haematobium but not Schistosoma mansoni infections in humans. American Journal of Tropical Medicine and Hygiene 55, 338–343.CrossRefGoogle Scholar
Agnew, A. M., Murare, H. M., Sandoval, S. N., de Jong, N., Krijer, F. W., Deelder, A. M. and Doenhoff, M. J. ( 1992). The susceptibility of adult schistosomes to immune attrition. Memórias do Instituto Oswaldo Cruz 87, 87–93.CrossRefGoogle Scholar
Bjorneboe, A. and Frandsen, F. ( 1979). A comparison of the characteristics of two strains of Schistosoma intercalatum Fisher, 1934 in mice. Journal of Helminthology 53, 195–203.CrossRefGoogle Scholar
Boulanger, D., Warter, A., Sellin, B., Lindner, V., Pierce, R. J., Chippaux, J-P. and Capron, A. ( 1999). Vaccine potential of a recombinant glutathione S-transferase cloned from Schistosoma haematobium in primates experimentally infected with an homologous challenge. Vaccine 17, 319–326. DOI: 10.1016/S0264-410X(98)00202-3.CrossRefGoogle Scholar
Bushara, H. O., Bashir, M. E., Malik, K. H., Mukhtar, M. M., Trottein, F., Capron, A. and Taylor, M. G. ( 1993). Suppression of Schistosoma bovis egg production in cattle by vaccination with either glutathione S-transferase or keyhole limpet haemocyanin. Parasite Immunology 15, 383–390.CrossRefGoogle Scholar
Bushara, H. O., Majid, B. Y. A., Majid, A. A., Khitma, I., Gameel, A. A., Karib, E. A., Hussein, M. F. and Taylor, M. G. ( 1983). Observations on cattle schistosomiasis in the Sudan, a study in comparative medicine. V. The effect of praziquantel therapy on naturally acquired resistance to Schistosoma bovis. American Journal of Tropical Medicine and Hygiene 32, 1370–1374.Google Scholar
Hagan, P., Blumenthal, U. J., Dunn, D., Simpson, A. J. G. and Wilkins, A. ( 1991). Human IgE, IgG4 and resistance to reinfection with Schistosoma haematobium. Nature, London 349, 243–245. DOI: 10·1038/349243a0.CrossRefGoogle Scholar
Johansen, M. V., Monrad, J. and Christensen, N. O. ( 1996 a). Effects of praziquantel on experimental Schistosoma bovis infection in goats. Veterinary Parasitology 62, 83–91.Google Scholar
Johansen, M. V., Monrad, J., Christensen, N. O. and Lindberg, R. ( 1996 b). Experimental Schistosoma bovis infection in goats. Pathological consequences of praziquantel treatment. Journal of Comparative Pathology 115, 1–11.Google Scholar
Johansen, M. V., Monrad, J., Christensen, N. O. and Lindberg, R. ( 1997). The impact of primary Schistosoma bovis infection on a subsequent challenge infection in goats. Journal of Parasitology 83, 242–246.CrossRefGoogle Scholar
Karanja, D. M. S., Hightower, A. W., Colley, D. G., Mwinzi, P. N. M., Galil, K., Andove, J. and Secor, W. E. ( 2002). Resistance to reinfection with Schistosoma mansoni in occupationally exposed adults and effect of HIV-1 co-infection on susceptibility to schistosomiasis: a longitudinal study. Lancet 360, 592–596.CrossRefGoogle Scholar
Kassuku, A. A., Christensen, N. O. and Nansen, P. ( 1986). Clinical pathology of Schistosoma bovis infection in goats. Research in Veterinary Science 40, 44–47.Google Scholar
Lindberg, R., Johansen, M. V., Monrad, J., Christensen, N. O. and Nansen, P. ( 1997). Experimental Schistosoma bovis infection in goats: the inflammatory response in the small intestine and liver in various phases of infection and reinfection. Journal of Parasitology 83, 454–459.CrossRefGoogle Scholar
Medhat, A., Shehata, M., Bucci, K., Mohamed, S., Dief, A. D. E., Badary, S., Galal, H., Nafeh, M. and King, C. L. ( 1998). Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment. Journal of Infectious Diseases 178, 512–519.CrossRefGoogle Scholar
Monrad, J., Christensen, N. O. and Nansen, P. ( 1991). Aquired resistance in goats following a single primary Schistosoma bovis infection. Acta Tropica 48, 69–77.Google Scholar
Monrad, J., Christensen, N. O., Nansen, P., Johansen, M. V. and Lindberg, R. ( 1995). Aquired resistance against Schistosoma bovis after single or repeated low-level primary infections in goats. Research in Veterinary Science 58, 42–45.CrossRefGoogle Scholar
Mutapi, F. ( 2001). Heterogeneities in anti-schistosome humoral responses following chemotherapy. Trends in Parasitology 17, 518–524. DOI: 10.1016/S1471-4922(01)02118-3.CrossRefGoogle Scholar
Roberts, M., Butterworth, A. E., Kimani, G., Kamau, T., Fulford, A. J. C., Dunne, D. W., Ouma, J. H. and Sturrock, R. F. ( 1993). Immunity after treatment of human schistosomiasis: association between cellular responses and resistance to reinfection. Infection and Immunity 61, 4984–4993.Google Scholar
SAS INSTITUTE ( 1999). SAS Version 8(2).
Taylor, M. G. ( 1987). Schistosomes of domestic animals: Schistosoma bovis and other animal forms. In Immune Responses in Parasitic infections, Vol II: Trematodes and Cestodes ( ed. Soulsby, E. J. L.), pp. 49–90. CRC Press, Boca Raton, Florida.
Tawfik, A. F. and Colley, D. G. ( 1986). Effects of anti-schistosomal chemotherapy on immune responses, protection and immunity. II. Concomitant immunity and immunization with irradiated cercariae. American Journal of Tropical Medicine and Hygiene 35, 110–117.Google Scholar
van Wyk, J. A., Heitmann, A. L. P. and van Rensburg, L. J. ( 1975). Studies on schistosomiasis. 7. A comparison of various methods for the infestation of sheep with Schistosoma mattheei. Onderstepoort Journal of Veterinary Research 42, 71–74.Google Scholar
Woolhouse, M. E. J. and Hagan, P. ( 1999). Seeking the ghost of worms past. Nature Medicine 5, 1225–1227. DOI: 10.1038/15169.CrossRefGoogle Scholar