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Thomsen-Friedenreich oncofetal antigen in Schistosoma mansoni: localization and immunogenicity in experimental mouse infection

Published online by Cambridge University Press:  21 September 2005

C. THORS ,
B. JANSSON ,
H. HELIN  and
E. LINDER
C. THORS
Affiliation:
Swedish Institute for Infectious Disease Control (SMI), S-171 82 Solna, Sweden Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, Box 280, S-171 77 Stockholm, Sweden
B. JANSSON
Affiliation:
BioInvent Production AB. Solveg 41, S-223 70 Lund, Sweden
H. HELIN
Affiliation:
Haartman Institute, Department of Pathology, Helsinki, Finland
E. LINDER
Affiliation:
Swedish Institute for Infectious Disease Control (SMI), S-171 82 Solna, Sweden Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, Box 280, S-171 77 Stockholm, Sweden
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Abstract

Our preliminary observation, that sera from schistosomiasis patients react with carcinomas, raised the possibility of antigenic cross-reactivity. We here extend this observation to show that mice experimentally infected with Schistosoma mansoni react with human urothelial and transitional bladder carcinomas and also with a gastric carcinoma cell line, AGS. To identify cross-reacting epitopes, we looked for the expression of carcinoma markers in schistosome worms and eggs using monoclonal antibodies against tumour antigens MUC1, Tn and TF (also known as the oncofetal Thomsen-Friedenreich antigen or T antigen). Immunohistochemical staining showed that the TF-epitope is present in adult intravascular S. mansoni worms and eggs deposited in tissues of infected animals. The localization of TF-immuno-reactive material in schistosomes was seen at the parasite surface between male and female worms and around trapped eggs in the liver. This localization is consistent with the antigen being secreted. Mice experimentally infected with S. mansoni, developed circulating antibodies against the TF-epitope (identified as Gal(beta1-3) GalNAc-O-R) as seen in ELISA using TF-expressing asialoglycophorin (AGP) as antigen. The observed anti-TF response in S. mansoni-infected mice reflects the complexity of host-parasite interactions in this infection.

Keywords

Schistosomiasiscarcinoma markeroncofetal antigenT-antigenTF-antigencarcinogenesisglycosylationglycotope
Type
Research Article
Information
Parasitology , Volume 132 , Issue 1 , January 2006 , pp. 73 - 81
Copyright
© 2005 Cambridge University Press

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