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Serotonin and its requirement for maintenance of contractility in muscle fibres isolated from Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

T. A. Day ,
J. L. Bennett  and
R. A. Pax
T. A. Day
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
J. L. Bennett
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
R. A. Pax
Affiliation:
Department of Zoology and Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48814, USA
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Summary

Muscle fibres isolated from adult Schistosoma mansoni contracted in a dose-dependent manner when exposed to elevated K+ with a maximum response obtained with 25 mM K+. These contractions were dependent on extracellular Ca2+ since Co2+ (5 mM) or nicardipine (1 μM) blocked the high K+ contractions. Serotonin (300 nM or higher) was required for maintenance of high K+ contractions. With concentrations of serotonin less than 300 nM the response was dose dependent. 5-Methoxytryptamine or α-methylserotonin at 1 μM as well as 10 μM tryptamine were able to substitute for serotonin, but 1 μM 5-carboxyamidotryptamine was ineffective. The order of potency for antagonists (10 μM) was: methiothepin > metergoline > Ly-278,584 = ketanserin. This pattern of responsiveness does not fit well with any known mammalian serotonin receptor subtype. Since forskolin, an adenylate cyclase activator, is able to mimic the action of serotonin and H89, a protein kinase inhibitor, is able to block the effect of serotonin, the effect of serotonin on contractility of the muscle may be via a cAMP-dependent pathway.

Keywords

Schistosoma mansoniplatyhelminthtrematodemuscleserotonin receptorcAMP
Type
Research Article
Information
Parasitology , Volume 108 , Issue 4 , May 1994 , pp. 425 - 432
Copyright
Copyright © Cambridge University Press 1994

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References

REFERENCES

Blair, K. L., Day, T. A., Lewis, M. C., Bennett, J. L. & Pax, R. A. (1991). Studies on muscle cells isolated from Schistosoma mansoni: a Ca2+-dependent K+ channel. Parasitology 102, 251–8.CrossRefGoogle Scholar
Bueding, E. & Mansour, J. M. (1957). The relationship between inhibition of phosphofructokinase activity and the mode of action of trivalent organic antimonials on Schistosoma mansoni. British Journal of Pharmacology 12, 159–65.Google ScholarPubMed
Day, T. A., Orr, N., Bennett, J. L. & Pax, R. A. (1993). Voltage-gated currents in muscle cells of Schistosoma mansoni. Parasitology 106, 471–7.CrossRefGoogle ScholarPubMed
Estey, S. & Mansour, T. (1987). Nature of serotonin-activated adenylate cyclase during development of Schistosoma mansoni. Molecular and Biochemical Parasitology 26, 47–60.CrossRefGoogle ScholarPubMed
Fetterer, R. H., Pax, R. A. & Bennett, J. L. (1977). Schistosoma mansoni: Direct method for simultaneous recording of electrical and motor activity. Experimental Parasitology 43, 286–94.CrossRefGoogle ScholarPubMed
Leake, L. D. & Walker, R. J. (1980). Invertebrate Neuropharmacology. Glasgow: Blackie.Google Scholar
Mansour, T. E. (1957). The effect of lysergic acid diethylamide, 5-hydroxytryptamine and related compounds on the liver fluke, Fasciola hepatica. British Journal of Pharmacology 12, 406–9.Google ScholarPubMed
Mansour, T. E. (1984). Serotonin receptors in parasitic worms. Advances in Parasitology 23, 2–36.Google ScholarPubMed
McNall, S. J. & Mansour, T. E. (1984). Novel serotonin receptors in Fasciola-characterization by studies on adenylate cyclase activation and 3H-LSD binding. Biochemical Pharmacology 33, 2789–97.CrossRefGoogle Scholar
Mellin, T., Busch, R., Wang, C. & Kath, G. (1983). Neuropharmacology of the parasitic trematode Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 32, 83–93.CrossRefGoogle ScholarPubMed
Northrup, J. K. & Mansour, T. E. (1978). Adenylate cyclase from Fasciola hepatica. I. Ligand specificity of adenylate cyclase-coupled serotonin receptors (804–819). Molecular Pharmacology 14, 804–19.Google Scholar
Richardson, B. P. & Hoyer, D. (1990). Selective agonists and antagonists at 5-hydroxytryptamine receptor subtypes. In Serotonin: From Cell Biology to Pharmacology and Therapeutics, (ed. Paoletti, R., Vanhoutte, P. M., Brunello, N. & Maggi, F. M.), pp. 265–76. Dordrecht: Kluwer Academic Publishers.CrossRefGoogle Scholar
Sukhdeo, S. & Sukhdeo, M. (1988). Immunohistochemical and electrochemical detection of serotonin in the nervous system of Fasciola hepatica, a parasitic flatworm. Brain Research 463, 57–62.CrossRefGoogle ScholarPubMed
Tembe, E. A., Holden-Dye, L., Smith, S. W., Jacques, P. A. & Walker, R. J. (1993). Pharmacological profile of the 5-hydroxytryptamine receptor of Fasciola hepatica body wall muscle. Parasitology 106, 67–73.CrossRefGoogle ScholarPubMed
Tomosky, T., Bennett, J. L. & Bueding, E. (1974). Tryptaminergic and dopaminergic responses of Schistosoma mansoni trematode; Schistosoma mansoni neurotransmitters serotonin. Journal of Pharmacology and Experimental Therapeutics 190, 260–71.Google Scholar
Van De Kar, L. (1991). Neuroendocrine pharmacology of serotoninergic (5-HT) neurons. Annual Review of Neuroscience 31, 289–310.Google Scholar
Waitz, J. A. (1964). Some aspects of the action of antimony on Schistosoma mansoni in vitro. Life Sciences 3, 337–80.Google ScholarPubMed
Walker, R. J. & Vehovszky, A. (1990). 5-Hydroxytryptamine (5-HT) receptor subtypes in invertebrates. In Serotonin: From Cell Biology to Pharmacology and Therapeutics, (ed. Paoletti, R., Vanhoutte, P. M., Brunello, N. & Maggi, F. M.), pp. 283–8. Dordrecht: Kluwer Academic Publishers.CrossRefGoogle Scholar
Witz, P., Amlaiky, N., Plassat, J.-L., Maroteaux, L., Borelli, E. & Hen, R. (1990). Cloning and characterization of Drosophila serotonin receptor that activates adenylate cyclase. Proceedings of the National Academy of Sciences, USA 87, 8940–4.CrossRefGoogle ScholarPubMed