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Genetic diversity of Babesia in Ixodes persulcatus and small mammals from North Ural and West Siberia, Russia

Published online by Cambridge University Press:  27 August 2010

V. A. RAR ,
T. I. EPIKHINA ,
N. N. LIVANOVA  and
V. V. PANOV
V. A. RAR*
Affiliation:
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russian Federation
T. I. EPIKHINA
Affiliation:
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russian Federation
N. N. LIVANOVA
Affiliation:
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russian Federation Institute of Systematics and Ecology of Animals, Siberian Branch, Russian Academy of Sciences, ul. Frunze 11, Novosibirsk, 630091 Russian Federation
V. V. PANOV
Affiliation:
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russian Federation Institute of Systematics and Ecology of Animals, Siberian Branch, Russian Academy of Sciences, ul. Frunze 11, Novosibirsk, 630091 Russian Federation
*
*Corresponding author: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, 630090 Russian Federation. Tel: +7 383 3635177. Fax: +7 383 3635164. E-mail: [email protected]
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Summary

Objective. The aim of this work was to study the prevalence and genetic diversity of Babesia in Ixodes persulcatus ticks and small mammals from Ural and Siberia in Russia. Methods. In total, 481 small mammals and 922 questing adult I. persulcatus from North Ural (Sverdlovsk region) and West Siberia (Novosibirsk region) were examined for the presence of Babesia by nested PCR based on the 18S rRNA gene. Results. Babesia microti of the ‘Munich’-type was found in 36·2% of blood samples of the small mammals from the Sverdlovsk region and B. microti of the ‘US’-type in 5·3% of the animals from the Novosibirsk region. Babesia DNA was not detected in 133 analysed I. persulcatus from the Sverdlovsk region; however, it was found in 24 of 789 ticks from the Novosibirsk region. Three distinct Babesia species were detected in I. persulcatus. B. microti ‘US’-type was identified in 10 ticks, Babesia closely related to B. divergens/B. capreoli in 2 ticks, and Babesia closely related to B. venatorum (EU1) in 12 ticks. Conclusion. To our knowledge, this is the first detection of Babesia sensu stricto in I. persulcatus ticks and of B. microti in I. persulcatus in the Asian part of Russia.

Keywords

Ixodes persulcatusBabesia spp.prevalencegenetic diversityNorth UralWest Siberia
Type
Research Article
Information
Parasitology , Volume 138 , Issue 2 , February 2011 , pp. 175 - 182
Copyright
Copyright © Cambridge University Press 2010

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