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Rosuvastatin reduced brain parasite burden in a chronic toxoplasmosis in vivo model and influenced the neuropathological pattern of ME-49 strain

Published online by Cambridge University Press:  18 November 2019

L. Nishi Open the ORCID record for L. Nishi [Opens in a new window] ,
P. L. Santana ,
F. F. Evangelista ,
L. F. Beletini ,
A. H. Souza ,
F. M. Mantelo ,
A. M. Souza-Kaneshima ,
I. N. Costa Open the ORCID record for I. N. Costa [Opens in a new window]  and
A. L. Falavigna-Guilherme
L. Nishi
Affiliation:
Postgraduate Program in Health Science, Department of Basic Health Sciences, State University of Maringá (UEM), Colombo Avenue, 5790. Bloco I90. CEP: 87020-900. Maringá, Paraná, Brazil
P. L. Santana
Affiliation:
Pathology Laboratory, Department of Basic Health Sciences, State University of Maringa, 5790 Colombo Avenue, Bloco I-90, CEP 87020-900, Maringá, Paraná, Brazil
F. F. Evangelista
Affiliation:
Postgraduate Program in Health Science, Department of Basic Health Sciences, State University of Maringá (UEM), Colombo Avenue, 5790. Bloco I90. CEP: 87020-900. Maringá, Paraná, Brazil
L. F. Beletini
Affiliation:
Postgraduate Program in Health Science, Department of Basic Health Sciences, State University of Maringá (UEM), Colombo Avenue, 5790. Bloco I90. CEP: 87020-900. Maringá, Paraná, Brazil
A. H. Souza
Affiliation:
Department of Basic Health Sciences, State University of Maringá (UEM), 5790 Colombo Avenue, Bloco I-90, CEP: 87020-900. Maringá, Paraná, Brazil
F. M. Mantelo
Affiliation:
Department of Basic Health Sciences, State University of Maringá (UEM), 5790 Colombo Avenue, Bloco I-90, CEP: 87020-900. Maringá, Paraná, Brazil
A. M. Souza-Kaneshima
Affiliation:
Pathology Laboratory, Department of Basic Health Sciences, State University of Maringa, 5790 Colombo Avenue, Bloco I-90, CEP 87020-900, Maringá, Paraná, Brazil
I. N. Costa
Affiliation:
Program of Experimental Pathology, Department of Pathological Sciences, State University of Londrina, Rodovia Celso Garcia Cid – PR 445 Km 380. Campus Universitário. Caixa Postal 10.011. CEP 86.057-970. Londrina, Paraná, Brazil
A. L. Falavigna-Guilherme*
Affiliation:
Parasitology Laboratory, Department of Basic Health Sciences, State University of Maringá, Colombo Avenue, 5790. Bloco I90. CEP: 87020-900. Maringá, Paraná, Brazil
*
Author for correspondence: A. L. Falavigna-Guilherme, E-mail: [email protected]
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Abstract

This study evaluated the effects of rosuvastatin in vivo on toxoplasmosis chronic infection. Thirty-five Swiss mice were orally infected (ME-49 strain). After 50 days, the mice were separated into five groups: GI – non-infected, GII – infected, GIII – infected and treated with pyrimethamine and sulfadiazine (12.5 + 50 mg kg−1 body weight day−1), GIV and GV – infected and treated with rosuvastatin 10 and 40 mg kg−1 body weight day−1, respectively. After 21 days, we collected blood, liver, lungs, femoral biceps and brain were removed for Toxoplasma gondii DNA quantification by qPCR and histopathological analysis. GIV and GV did not present premature death or clinical changes, and the hepatic enzyme levels were lower compared to GI. Toxoplasma gondii DNA was detected mainly in brain and muscle, but the parasite load was significantly lower in GV compared to GII brains (P < 0.05). Histopathological changes were observed in brains, with T. gondii cysts as well as an inflammatory condition, including necrosis areas in GII and GIII. These data confirm active infection with tissue injury. This inflammatory condition was attenuated in the groups treated with rosuvastatin, especially R40 (GV). Our findings demonstrated the in vivo action of rosuvastatin in reducing cerebral parasitic load and indicate that this drug may interfere in chronic toxoplasmosis.

Keywords

StatinsSwiss micetoxoplasmosis treatment
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 303 - 309
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Copyright
Copyright © Cambridge University Press 2019

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