Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-28T16:47:56.077Z Has data issue: false hasContentIssue false
  • English
  • Français

Infection of mammalian cells with Theileria species*

Published online by Cambridge University Press:  06 April 2009

D. A. Stagg ,
A. S. Young ,
B. L. Leitch ,
J. G. Grootenhuis  and
T. T. Dolan
D. A. Stagg
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
B. L. Leitch
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
J. G. Grootenhuis
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
T. T. Dolan
Affiliation:
Protozoology Division, Veterinary Research Department, Kenya Agricultural Research Institute, Muguga, P.O. Box 32, Kikuyu, Kenya
Get access Rights & Permissions [Opens in a new window]

Summary

Experiments were carried out to determine the susceptibility of mammalian cells to infection with different species of Theileria in vitro. Sporozoites of Theileria parva (parva), Theileria parva (lawrencei) and Theileria taurotragi were isolated from Rhipicephalus appendiculatus ticks by grinding infected ticks in medium, filtering the suspension and concentrating by centrifugation. The sporozoites were used in attempts to infect in vitro peripheral blood leucocytes harvested from 16 different mammalian species which included 12 species of Bovidae from 6 different sub-families. The technique was shown to be both sensitive and reproducible. The sporozoites of T. parva (parva) infected and transformed cells from 2 species of the sub-family Bovinae, the two cattle types and African buffalo. Theileria parva (lawrencei) infected and transformed cells from the two cattle types, African buffalo and Defassa waterbuck. Theileria taurotragi sporozoites infected in vitro cells from 11 different species of Bovidae which were members of 6 sub-families; Bovinae, Tragelaphinae, Reduncinae, Alcelaphinae, Antilopinae and Caprinae. Transformed lymphoblastoid cell lines were established from 7 of the species infected. Sporozoite attachment and infection was not observed with non-susceptible bovid host cells, nor were any of the non-bovid leucocytes infected by the parasites. The host range observed in this study corresponded to the known host range in vivo.

Type
Research Article
Information
Parasitology , Volume 86 , Issue 2 , April 1983 , pp. 243 - 254
Copyright
Copyright © Cambridge University Press 1983

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bailey, K. P. (1960). Notes on the rearing of Rhipicephalus appendiculatus and their infection with Theileria parva for experimental transmission. Bulletin of Epizootic Diseases of Africa 8, 33–43.Google Scholar
Barnett, S. F. & Brocklesby, D. W. (1966). The passage of Theileria lawrencei (Kenya) through cattle. British Veterinary Journal 122, 369–409.Google Scholar
Blewett, D. A. & Branagan, D. (1973). The demonstration of Theileria parva infection in intact Rhipicephalus appendiculatus salivary glands. Tropical Animal Health and Production 5, 27–34.CrossRefGoogle Scholar
Brocklesby, D. W. (1964). Parasites of the family Theileriidae of the African buffalo occurring in East Africa. Dr. Med. Vet. thesis, University of Zurich.Google Scholar
Brocklesby, D. W. & Vidler, B. O. (1966). Haematozoa found in wild members of the order Artiodactyla in East Africa. Bulletin of Epizootic Diseases of Africa 14, 285–99.Google ScholarPubMed
Brown, C. G. D. (1979). Progation of Theileria. In Practical Tissue Culture Applications (ed. Maramorosch, K. and Hirumi, H.), pp. 223–45. New York: Academic Press.Google Scholar
Brown, C. G. D. & Gray, M. A. (1981). Infection of bovine fibroblastic cell lines with Theileria parva and T. annulata. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 323–4.Google Scholar
Brown, C. G. D., Stagg, D. A., Purnell, R. E., Kanhai, G. K. & Payne, R. C. (1973). Infection and transformation of bovine lymphoid cells in vitro by infective particles of Theileria parva. Nature, London 245, 101–2.CrossRefGoogle ScholarPubMed
Chema, S. & Brocklesby, D. W. (1981). Epidemiology: appraisal and future perspectives. In Advances in the Control of Theileriosis (ed. Irvin, A. D.Cunningham, M. P. and Young, A. S.), pp. 100–3. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Cunningham, M. P., Brown, C. G. D., Burridge, M. J. & Purnell, R. E. (1973). Cryo-preservation of infective particles of Theileria parva. International Journal for Parasitology 3, 583–7.CrossRefGoogle Scholar
Fawcett, D. W., Doxsey, S., Stagg, D. A. & Young, A. S. (1982). The entry of sporozoites of Theileria parva into bovine lymphocytes in vitro. Electron microscopic observations. European Journal of Cell Biology 27, 10–21.Google ScholarPubMed
Grootenhuis, J. G. (1979). Theileriosis of wild Bovidae in Kenya with special reference to the eland (Taurotragus oryx). Vet. Med. thesis, University of Utrecht.Google Scholar
Grootenhuis, J. G. & Young, A. S. (1981). The involvement of wildlife in Theileria infections of domestic animals in East Africa. In Advances in the Control of Theileriosis (ed. Irvin, A. D.Cunningham, M. P. and Young, A. S.), pp. 71–73. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Grootenhuis, J. G., Karstad, L. & Drevemo, S. A. (1976). Experience with drugs for capture and restraint of wildebeest, impala, eland and hartebeest in Kenya. Journal of Wildlife Diseases 12, 435–43.CrossRefGoogle ScholarPubMed
Grootenhuis, J. G., Young, A. S. & Uilenberg, G. (1981). The relationship between Theileria taurotragi from eland and Theileria species (Idobogo) from cattle. Veterinary Parasitology 8, 39–47.CrossRefGoogle Scholar
Hulliger, L., Wilde, J. K. H., Brown, C. G. D. & Turner, L. (1964). Mode of multiplication of Theileria in culture of bovine lymphocytic cells. Nature, London 203, 728–30.CrossRefGoogle ScholarPubMed
Kurtti, T. J., Munderloh, U. G., Irvin, A. D. & Büscher, G. (1981). Theileria parva: Early events in the development of bovine lymphoblastoid cell lines persistently infected with macroschizonts. Experimental Parasitology 52, 280–90.CrossRefGoogle ScholarPubMed
Lewis, E. A. (1943). East Coast fever and the African buffalo, the eland and the bushbuck. The East African Agricultural Journal 9, 90–2.CrossRefGoogle Scholar
Malmquist, W. A. & Brown, C. G. D. (1974). Establishment of Theileria parat-infected lymphoblastoid cell lines using homologous feeder layers. Research in Veterinary Science 16, 134–5.CrossRefGoogle ScholarPubMed
Malmquist, W. A., Nyindo, M. B. A. & Brown, C. G. D. (1970). East Coast fever; cultivation in vitro of bovine spleen cell lines infected and transformed by Theileria parva. Tropical Animal Health and Production 2, 139–45.CrossRefGoogle Scholar
Malmquist, W. A., van der Maaten, M. J. & Boothe, A. D. (1969). Isolation, immunodiffusion, immunofluorescence and electron microscopy of a syncytial virus of lymphosarcomatous and addarently normal cattle. Cancer Research 29. 188–200.Google ScholarPubMed
Martin, H. & Bbocklesby, D. W. (1960). A new parasite of the eland. Veterinary Record 72, 331–2.Google Scholar
Morrison, W. I., Buscher, G., Emery, D. L., Nelson, R. T. & Murray, M. (1981). The kinetics of infection with Theileria parva in cattle and the relevance to the development of immunity. In Advances in the Control of Theileriosis (ed. Irvin, Cunningham, M. P. and Young, A. S.), pp. 311–26. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Purnell, R. E., Radley, D. E. & Cunningham, M. P. (1974). A note on the susceptibility of sheep and goats to East Coast fever. Veterinary Record 94, 278.CrossRefGoogle ScholarPubMed
Stagg, D. A. (1981). The infectivity of Theileria sporozoites for bovid leucocytes in vitro. In Advances in the Control of Theileriosis (ed. Irvin, A. D.Cunningham, M. P. and Young, A. S.), pp. 132–5. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Stagg, D. A., Brown, C. G. D., Crawford, J. G., Kanhai, G. K. & Young, A. S. (1974). In vitro cultivation of Theileria lawrencei infected lymphoblastoid cell lines derived from a buffalo (Syncerus caffer). Research in Veterinary Science 16, 125–7.CrossRefGoogle ScholarPubMed
Stagg, D. A., Dolan, T. T., Leitch, B. L. & Young, A. S. (1981). The initial stages of infection of cattle cells with Theileria parva sporozoites in vitro. Parasitology 83, 191–7.CrossRefGoogle ScholarPubMed
Stagg, D. A., Kanhai, G. K., Young, A. S. & Brown, C. G. D. (1976). The establishment of Theileria infected cell lines from an eland. (Taurotragus oryx, Lydekker 1906). Research in Veterinary Science 20, 122–6.CrossRefGoogle ScholarPubMed
Stagg, D. A., Chasey, D., Young, A. S., Morzaria, S. P. & Dolan, T. T. (1980). Synchronization of the division of Theileria macroschizonts and their mammalian host cells. Annals of Tropical Medicine and Parasitology 74, 263–6.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D.Cunningham, M. P. and Young, A. S.), pp. 4–37. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Young, A. S. (1981). The epidemiology of theileriosis in East Africa. In Advances in the Control of Theileriosis (ed. Irvin, A. D.Cunningham, M. P. and Young, A. S.), pp. 38–55. The Hague: Martinus Nijhoff Publishers.CrossRefGoogle Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M. & Irvin, A. D. (1973). Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. International Journal for Parasitology 3, 723–8.CrossRefGoogle ScholarPubMed
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Payne, R. C. & Purnell, R. E. (1977 a). Establishment of an experimental field population of Theileria lawrencei-infected ticks maintained by African buffalo (Syncerus caffer). Journal of Parasitology 63, 903–7.CrossRefGoogle ScholarPubMed
Young, A. S., Grootenhuis, J. G., Kimber, C. D., Kanhai, G. K. & Stagg, D. A. (1977 b) Isolation of a Theileria species from eland (Taurotragus oryx) infective for cattle. Tropenmedizin und Parasitologic 28, 184–94.Google ScholarPubMed
Young, A. S. & Leitch, B. L. (1981). Epidemiology of East Coast fever: Some effects of temperature on the development of Theileria parva in the tick vector, Rhipicephalus appendiculatus. Parasitology 83, 199–211.CrossRefGoogle ScholarPubMed
Young, A. S. & Purnell, R. E. (1973). Transmission of Theileria lawrencei (Serengeti) by ixodid tick, Rhipicephalus appendiculatus. Tropical Animal Health and Production 5, 146–52.CrossRefGoogle ScholarPubMed