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Protein/Polymer Hybrid Biomimetic Valves

Published online by Cambridge University Press:  11 February 2011

Dean Ho ,
Jacob J. Schmidt  and
Carlo D. Montemagno
Dean Ho
Affiliation:
UCLA Department of Bioengineering Los Angeles, CA 90095, U.S.A.
Jacob J. Schmidt
Affiliation:
UCLA Department of Bioengineering Los Angeles, CA 90095, U.S.A.
Carlo D. Montemagno
Affiliation:
UCLA Department of Bioengineering Los Angeles, CA 90095, U.S.A.
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Abstract

Membrane channel and pore proteins represent a versatile class of molecules that offer opportunities to serve as engineered nano-valves. We are inserting OmpF, a voltage-gatable pore protein, into planar membranes for applications in microfluidics and sensing. To emulate and improve upon natural membranes, we have selected triblock copolymers containing UV cross-linkable end groups. We are developing a microfabricated version of a conventional black lipid membrane/copolymer characterization apparatus which will enable us to rapidly analyze composite membrane properties as a function of area and applied electrical bias. We show that only slight deviations of conventional planar lipid membrane formation procedures with triblock copolymers are sufficient for polymer monolayer membrane formation. We report the results of the preliminary tests on polymer membrane formation and stability as well as the construction of the microfabricated device.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 735: Symposium C – Bioinspired Nanoscale Hybrid Systems , 2002 , C4.5
Copyright
Copyright © Materials Research Society 2003

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References

REFERENCES

1. Saint, N., Lou, K.L., Widmer, C., Luckey, M., Schirmer, T., Rosenbusch, J.P., J. Biol. Chem. 271 20676–20680 (1996)Google Scholar
2. Muller, D. and Engel, A., J. Mol. Bio. 285 1347–1351 (1999)Google Scholar
3. Montal, M. and Mueller, P., Proc. Nat. Acad. Sci. USA 69 3561–3566 (1972)Google Scholar
4. Nardin, C., Winterhalter, M., Meier, W., Langmuir 16, 7708–7712 (2000)Google Scholar
5. Meier, W., Nardin, C., Winterhalter, M., Angew. Chem. Int. Ed. 39 4599–4602 (2000)Google Scholar
6. Prilipov, A., Phale, P., Van Gelder, P., Rosenbusch, J.P., Koebnik, R., FEMS Microbiol. Lett. 163 65–72 (1998)Google Scholar
7. Alvarez, O., in Ion Channel Reconstitution, edited by Miller, C. (Plenum Press, New York, 1986, p.115–139)Google Scholar
8. Winterhalter, M., Klotz, K., Benz, R., in Electromanipulation of Cells, edited by Zimmerman, U., Neil, G.A. (CRC Press, Boca Raton, 1995, p.137)Google Scholar
9. Diederich, A., Strobel, M., Meier, W., Winterhalter, M., J. Phys. Chem. B 103, 1402–1407 (1999)Google Scholar
10. Pantoja, R., Sigg, D., Blunck, R., Bezanilla, F., Heath, J., Biophys. J. 81 2389–2394 (2001)Google Scholar