Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-28T10:36:01.701Z Has data issue: false hasContentIssue false
  • English
  • Français

Using Peptide Hetero-assembly to Trigger Physical Gelation and Cell Encapsulation

Published online by Cambridge University Press:  01 February 2011

Andreina Parisi-Amon ,
Cheryl Wong Po Foo ,
Ji Seok Lee ,
Widya Mulyasasmita  and
Sarah Heilshorn
Andreina Parisi-Amon
Affiliation:
[email protected], Stanford University, Bioengineering, Stanford, California, United States
Cheryl Wong Po Foo
Affiliation:
[email protected], Stanford University, Materials Science and Engineering, Stanford, California, United States
Ji Seok Lee
Affiliation:
[email protected], Stanford University, Materials Science and Engineering, Stanford, California, United States
Widya Mulyasasmita
Affiliation:
[email protected], Stanford University, Bioengineering, Stanford, California, United States
Sarah Heilshorn
Affiliation:
[email protected], Stanford University, Materials Science and Engineering, 476 Lomita Mall, McCullough Building, Room 246, Stanford, California, 94305-4045, United States
Get access

Abstract

Stem cell transplantation holds tremendous potential for the treatment of various trauma and diseases. However, the therapeutic efficacy is often limited by poor and unpredictable post-transplantation cell survival. While hydrogels are thought to be ideal scaffolds, the sol-gel phase transitions required for cell encapsulation within commercially available biomatrices such as collagen and Matrigel often rely on non-physiological environmental triggers (e.g., pH and temperature shifts), which are detrimental to cells. To address this limitation, we have designed a novel class of protein biomaterials: Mixing-Induced Two-Component Hydrogels (MITCH) that are recombinantly engineered to undergo gelation by hetero-assembly upon mixing at constant physiological conditions, thereby enabling simple, biocompatible cell encapsulation and transplantation protocols. Building upon bio-mimicry and precise molecular-level design principles, the resulting hydrogels have tunable viscoelasticity consistent with simple polymer physics considerations. MITCH are reproducible across cell-culture systems, supporting growth of human endothelial cells, rat mesenchymal stem cells, rat neural stem cells, and human adipose-derived stem cells. Additionally, MITCH promote the differentiation of neural progenitors into neuronal phenotypes, which adopt a 3D-branched morphology within the hydrogels.

Keywords

biomaterialbiomimetic (assembly)polymer
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1272: Symposia KK/LL/NN/OO/PP – Integrated Miniaturized Materials-From Self-Assembly to Device Integration , 2010 , 1272-NN05-08
Copyright
Copyright © Materials Research Society 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1 Willerth, SM., Sakiyama-Elbert, SE, Adv. Drug Delivery Rev Rev. 60, 263 (2008).Google Scholar
2 Bjorklund, A., et al, Brain Res Res. 886, 82 (2000).Google Scholar
3 Siatskas, C., Bernand, CC., Current Molecular Medicine 9, 992 (2009).Google Scholar
4 Kordower, JH., et al, Mov. Disord Disord. 13, 383 (1998).Google Scholar
5 Kordower, JH., et a., N. Engl. J. Med. 332, 1118 (995).Google Scholar
6 Cao, F., et al, J. Tissue Eng. Regen. Med Med. 1, 465 (2007).Google Scholar
7 Laflamme, MA., et al, Nat. Biotechnol Biotechnol. 25, 1015 (2007).Google Scholar
8 Brandl, F., Sommer, F., Goepferich, A., Biomaterials 28, 134 (2007).Google Scholar
9 Petka, WA., et al, Sci Science 281, 389 (1998).Google Scholar
10 Capito, RM., et al, Science 319, 1812 (2008).Google Scholar
11 -Buterick, L. Haines, et al, Poc. Natl. Acad. Sci. USA 104, 7791 (2007).Google Scholar
12 Pochan, DJ., et al, J. Am. Chem. Soc. 125, 11802 (2003).Google Scholar
13 Niece, KL, et al, J. Am. Chem. Soc. 125, 7146 (2003).Google Scholar
14 Gillette, BM., et al, Nat. Mater. 7, 636 (2008).Google Scholar
15 Wang, S., et al, Tissue Eng. Part A 14, 227 (2008).Google Scholar
16 Teixeira, AI., Duckworth, JK., Hermanson, O., Cell Res. 17, 56 (2007).Google Scholar
17 Foo, CTS. Wong Po, et al, Poc. Natl. Acad. Sci. USA 106, 22067 (2009).Google Scholar
18 Crocker, JC., Grier, DG, J. C Colloid Interface Sci. 179, 298 (1996).Google Scholar
19 Huang, X., Nat. Struct Biol. 7, 634 (2000).Google Scholar
20 Flory, PJ., J. Am. Chem. Soc. 63, 3083 (1941).Google Scholar
21 KAnelis, V., Rotin, D., Forman-Kay, JD., Nat. Struct. Biol. 8, 407 (2001).Google Scholar
22 Russ, WP., et al, Nature 437, 579 (2005).Google Scholar
23 Zaman, MH., et al, Poc. Natl. Acad. Sci. USA 103, 10889 (2006).Google Scholar