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Interactions of Carbon Nanomaterials With Mammalian Cells

Published online by Cambridge University Press:  01 February 2011

Pavan M. V. Raja ,
Jennifer Connolley ,
Lijie Ci ,
Gopal P. Ganesan ,
Pulickel M. Ajayan ,
Omkaram Nalamasu  and
Deanna M. Thompson
Pavan M. V. Raja
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Chemical and Biological Engineering, CII 9015 - CIE, 110 8th Street, Troy, NY, 12180, United States
Jennifer Connolley
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Biomedical Engineering, Troy, NY, 12180, United States
Lijie Ci
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Materials Science and Engineering, Troy, NY, 12180, United States
Gopal P. Ganesan
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Center for Integrated Electronics, Troy, NY, 12180, United States
Pulickel M. Ajayan
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Materials Science and Engineering, Troy, NY, 12180, United States
Omkaram Nalamasu
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Center for Integrated Electronics, Troy, NY, 12180, United States
Deanna M. Thompson
Affiliation:
[email protected], Rensselaer Polytechnic Institute, Biomedical Engineering, Troy, NY, 12180, United States
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Abstract

Despite their diverse application potential, carbon nanotubes (CNT) have adverse effects in vitro, and in vivo. Previous research has focused on the in vitro cytotoxic impact of CNT aggregates and associated nanoparticulate impurities. In this study, we compared the single-walled carbon nanotube (SWNT) aggregates, and their associated finely dispersed, non-aggregated carbon nanomaterials on rat aortic smooth muscle cells (SMC), through filtration of the aggregates from the CNT-treated cell culture media. In general, our research shows that the removal of single-walled carbon nanotube (SWNT) aggregates from cell culture test media inhibited the growth in SMC to a lower extent than the corresponding unfiltered media at pre-filtered SWNT dosages below 0.10 mg/ml. We also found suspended nanoparticles (likely amorphous and graphitic carbon associated with the SWNT) and a small quantity of SWNT in the filtered media may have contributed to the observed cell growth inhibition by the filtered media. In addition, we compared the effect of SWNT, a nano-sized material, with activated carbon (AC), a nanoporous, microparticulate material, on SMC growth. AC (0.1 mg/ml) was found to be less inhibitory to SMC growth than the SWNT aggregates and suspended matter (0.1 mg/ml), potentially implying an inverse proportionality between carbon nanomaterial size regimes and cell growth inhibition.

Keywords

nanoscalebiologicalbiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 951: Symposium E – Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection , 2006 , 0951-E04-08
Copyright
Copyright © Materials Research Society 2007

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References

REFERENCES

1. Ajayan, P. M., Nanotubes from carbon, Chem. Rev. 99, pp.1787–1799 (1999).Google Scholar
2. Pantarotto, D. et al. Functionalized carbon nanotubes for plasmid DNA gene delivery, Angew. Chem. Int. Ed. 43, pp.5242–5246 (2004).Google Scholar
3. Nimmagadda, A. et al, Chemical modification of SWNT alters in vitro cell-SWNT interactions, J. Biomed. Mat. Res. 76, pp.614–625 (2006).Google Scholar
4. Shvedova, A. A., et al, Exposure to carbon nanotube material: assessment of nanotube cytotoxicity using human keratinocyte Cells, J. Tox. & Env. Health. 66, pp.1909–1926 (2003).Google Scholar
5. Monteiro-Riviere, N. A., et al, Multi-walled carbon nanotube interactions with human epidermal keratinocytes, Tox. Lett. 155, pp.377–384 (2005).Google Scholar
6. Magrez, A. et al, Cellular toxicity of carbon-based nanomaterials, Nano Lett. 6, pp.1121–1125 (2006).Google Scholar
7. Hill, J. B. et al. Efficacy of activated charcoal hemoperfusion in removing lethal doses of barbiturates and salicylate from the blood of rats and dogs, Clin. Chem. 22, pp.754–760 (1976).Google Scholar
8. Chang, T. M. S., and Malave, N., The development and first clinical use of semipermeable microcapsules (artificial cells) as a compact artificial kidney, Clin. Apheresis. 4, pp.108–116 (2000).Google Scholar