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Enhancing cell culture in magnetic vesicle gels

Published online by Cambridge University Press:  01 February 2011

Felicity Leng ,
Julie E Gough  and
Simon J Webb
Felicity Leng
Affiliation:
[email protected], University of Manchester, School of Chemistry and MIB, Manchester, United Kingdom
Julie E Gough
Affiliation:
[email protected], University of Manchester, School of Materials, Manchester, United Kingdom
Simon J Webb
Affiliation:
[email protected], University of Manchester, School of Chemistry and MIB, Manchester, United Kingdom
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Abstract

Several different hydrogel compositions have been incorporated into magnetic vesicle gels and the resulting “smart” biomaterials assessed as cell culture scaffolds. The compatibility of these hydrogels with the “smart” component of these biomaterials, thermally sensitive vesicles (TSVs) crosslinked by magnetic nanoparticles, was assessed by the leakage of fluorescent 5/6-carboxyfluorescein from the TSVs under cell culture conditions. Subsequently the ability of the hydrogels to support 3T3 fibroblast and chondrocyte viability was assessed. These studies revealed that alginate-based gels were the most compatible with both the TSVs and the cultured cells, with an alginate:fibronectin mix proving to be the most versatile. Nonetheless these studies also suggest that TSV composition needs to be modified to improve the performance of these “smart” cell culture scaffolds in future applications.

Keywords

magnetic propertiesbiomimetic (assembly)biomaterial
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1272: Symposia KK/LL/NN/OO/PP – Integrated Miniaturized Materials-From Self-Assembly to Device Integration , 2010 , 1272-PP07-02
Copyright
Copyright © Materials Research Society 2010

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References

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