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Carbohydrate-Carbohydrate Recognition Promotes Cell Adhesion

Published online by Cambridge University Press:  01 February 2011

Christine Gourier ,
Eric Perez ,
Yongmin Zhang  and
Pierre Sinaÿ
Christine Gourier
Affiliation:
Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, UMR 8550 associée au CNRS et aux universités Paris 6 et Paris 7.
Eric Perez
Affiliation:
Laboratoire de Physique Statistique de l'Ecole Normale Supérieure, UMR 8550 associée au CNRS et aux universités Paris 6 et Paris 7.
Yongmin Zhang
Affiliation:
Département de Chimie de l'Ecole Normale Supérieure, UMR 8642 associée au CNRS, 75231 Paris Cedex 05, France.
Pierre Sinaÿ
Affiliation:
Département de Chimie de l'Ecole Normale Supérieure, UMR 8642 associée au CNRS, 75231 Paris Cedex 05, France.
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Abstract

Recently, carbohydate-carbohydrate recognition has emerged as a new type of interaction in cell adhesion processes. One of these carbohydrates, the LewisX determinant (LeX), has been shown to be involved in murine embryogenesis. Here we confirm the existence of this specific interaction by measuring the adhesion between giant vesicles functionalised with synthetic Lex bearing lipids providing to the LeXdeterminent a high orientational freedom. This was obtained by micropipette aspiration and contact angle measurements. By using a simple model involving the several contributions to the adhesion free energy, specific and non specific interactions could be separated and quantified. In a second step, using natural Lex bearing sphingolipids, we could underscore the high sensitivity of LeX-LeX recognition to molecular structure and prove that the possible orientations provided by the natural LeX bearing molecules not only allow but strongly favor LeX-LeX recognition.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 872: Symposium J – Micro- and Nanosystems—Materials and Devices , 2005 , J12.4
Copyright
Copyright © Materials Research Society 2005

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