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Bacterial attachment on poly[acrylonitrile-co-(2-methyl-2-propene-1-sulfonic acid)] surfaces

Published online by Cambridge University Press:  27 June 2013

Petra Landsberger ,
Viola Boenke ,
Anna A. Gorbushina ,
Karsten Rodenacker ,
Benjamin F. Pierce ,
Karl Kratz  and
Andreas Lendlein
Petra Landsberger
Affiliation:
Free University of Berlin and Federal Institute for Materials Research and Testing (BAM), Unter den Eichen 87, 12205 Berlin, Germany
Viola Boenke
Affiliation:
Free University of Berlin and Federal Institute for Materials Research and Testing (BAM), Unter den Eichen 87, 12205 Berlin, Germany
Anna A. Gorbushina
Affiliation:
Free University of Berlin and Federal Institute for Materials Research and Testing (BAM), Unter den Eichen 87, 12205 Berlin, Germany
Karsten Rodenacker
Affiliation:
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Biomathematics and Biometry, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany
Benjamin F. Pierce
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany
Karl Kratz
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany
Andreas Lendlein
Affiliation:
Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany
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Abstract

The influence of material properties on bacterial attachment to surfaces needs to be understood when applying polymer-based biomaterials. Positively charged materials can kill adhered bacteria when the charge density is sufficiently high [1] but such materials initially increase the adherence of some bacteria such as Escherichia coli [2]. On the other hand, negatively charged materials have been shown to inhibit initial bacterial adhesion [3], but this effect has only been demonstrated in relatively few biomaterial classes and needs to be evaluated using additional systems. Gradients in surface charge can impact bacterial adhesion and this was tested in our experimental setup.

Moreover, the evaluation of bacterial adhesion to biomaterials is required to assess their potential for biological applications. Here, we studied the bacterial adhesion of E. coli and Bacillus subtilis on the surfaces of acrylonitrile-based copolymer samples with different amounts of 2-methyl-2-propene-1-sulfonic acid sodium salt (NaMAS) comonomer. The content related to NaMAS based repeating units nNaMAS varied in the range from 0.9 to 1.5 mol%.

We found a reduced colonized area of E. coli for NaMAS containing copolymers in comparison to pure PAN materials, whereby the bacterial colonization was similar for copolymers with different nNaMAS amounts. A different adhesion behavior was obtained for the second tested organism B. subtilis, where the implementation of negative charges into PAN did not change the overall adhesion pattern. Furthermore, it was observed that B. subtilis adhesion was significantly increased on copolymer samples that exhibited a more irregular surface roughness.

Keywords

biomaterialbiologicalpolymer
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1569: Symposium NN – Advanced Materials for Biological and Biomedical Applications , 2013 , pp. 85 - 90
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Murata, H., Koepsel, R. R., Matyjaszewski, K., Russell, A.J., Biomaterials 28, 4870 (2007).CrossRefGoogle Scholar
Harkes, G., Feijen, J., Dankert, J., Biomaterials 12, 853 (1991).CrossRefGoogle Scholar
Lawrence, N. N., Wells-Kinsbury, J. M., Ihrig, M. M., Fangman, T. E., Namavar, F., Cheung, C. L., Langmuir 28, 4301 (2012).CrossRefGoogle Scholar
Scharnagl, N., Hiebl, B., Trescher, K., Zierke, M., Behl, M., Kratz, K., Jung, F., Lendlein, A., Clinical Hemorheology and Microcirculation 52, 295–311 (2012).Google Scholar
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T., Molecular Systems Biology 2:2006.0007 (2006).CrossRefGoogle Scholar
Neidhardt, F. C., Bloch, P. L., Smith, D. F., Journal of Bacteriology 119 (3), 736–747 (1974).Google Scholar
Rasband, W.S.. ImageJ, U.S. National Institutes of Health, Bethesda, Maryland, USA,imagej.nih.gov/ij/ (1997—2012).Google Scholar
R Core Team, R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL http://www.R-project.org/ (2013).Google Scholar