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TEM of Bacteriophages Found in Marine Sources

Published online by Cambridge University Press:  14 March 2018

Adella Fejeran
Affiliation:
Mount Miguel High School, Spring Valley, CA
Jesus Polanco
Affiliation:
Hilltop High School, Chula Vista, CA
Gabriel Lander
Affiliation:
National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA
Teddy Ajero Jr
Affiliation:
National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA
Bridget Carragher
Affiliation:
National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA
Clinton S. Potter*
Affiliation:
National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA

Abstract

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We describe here a project to illustrate the diversity and abundance of marine bacteriophages undertaken by two high school students participating in The Scripps Research Institute's High School Student Research Education Program. The students were interns in the Automated Molecular Imaging group over the summer of 2007, during which time they acquired high magnification transmission electron microscopy images of bacteriophage filtered from samples collected from nearby marine waters. The basic protocols for sample collection, grid preparation, and electron microscopy imaging are described in this manuscript along with some of the images of the bacteriophages.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2007

References

Ackermann, H.W., 2001. Frequency of morphological phage descriptions in the year 2000. Archives of Virology 146: 843857.Google Scholar
Bergh, O., Borsheim, K.Y., Bratbak, G., and Heldal, M., 1989. High abundance of viruses found in aquatic environments. Nature 340: 467468.CrossRefGoogle ScholarPubMed
Breitbart, M., 2002. Genomic analysis of uncultured marine viral communities. Proc. Natl Acad. Sci. USA 99: 1425014255.Google Scholar
Breitbart, M., 2004. Diversity and population structure of a near-shore marine-sediment viral community. Proc. R. Soc. Lond. B 271: 565574.Google Scholar
Breitbart, M., Miyake, J.H., and Rohwer, F., 2004. Global distribution of nearly identical phage-encoded DNA sequences. FEMS Microbiol. Lett. 236: 249256.Google Scholar
Hennes, K.P., and Suttle, C.A., 1995. Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol. Oceanogr. 40: 10501055.Google Scholar
Rohwer, F., 2000. The complete genomic sequence of the marine phage Roseophage SIO1 shares homology with nonmarine phages. Limnol. Oceanogr. 45: 408418.CrossRefGoogle Scholar
Suloway, C., Pulokas, J., Fellmann, D., Cheng, A., Guerra, F., Quispe, J., Stagg, S., Potter, C.S., and Carragher, B., 2005. Automated molecular microscopy: the new Leginon system. J Struct Biol 151: 4160.CrossRefGoogle ScholarPubMed
Suttle, C.A., 2005. Viruses in the sea. Nature 437: 356361.Google Scholar