A Novel Approach to Reembedding Thick Resin Sections for Ultramicrotomy

Extract

In transmission electron microscopy (TEM), it is often desirable to focus our attention on certain cells or structures that are sparsely distributed throughout a large tissue block. One method that is often used is to serially section through a large tissue block and then scan each thin section on the TEM for the cell or structure of interest. A much simpler and far less tedious method is to cut thick sections of the resin block, screen the sections using light microscopy for the cells or structures of interest and then somehow reembed only those sections with the cell or structure of interest so that they can be thin sectioned for TEM.