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Mounting Media and Antifade Reagents

Published online by Cambridge University Press:  14 March 2018

Tony J. Collins
Tony J. Collins*
Affiliation:
Wright Cell Imaging Facility, Toronto Western Research Institute
*
[email protected]

Extract

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In the biomedical sciences, samples are mounted in a wide variety of media for examination by microscope. There are a wide variety of mounting media available with a correspondingly wide range of properties. Using the incorrect mounting medium may cause signal loss and optical aberrations; the correct mounting medium avoids such aberrations and preserves fluorescence signal with “anti-fading” properties. This article introduces mounting media for fluorescence microscopy, providing descriptions of their constituents and their properties, as well as accounts of users' experience

More detailed reviews of antifade reagents have been published by Ono et al. and Longin et al.. Papers describing the effect of refractive index (RI) mismatch have been published by Diaspro et al. and Hell et al..

Type
Research Article
Information
Microscopy Today , Volume 14 , Issue 1 , January 2006 , pp. 34 - 39
Copyright
Copyright © Microscopy Society of America 2006

References

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