Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-08T02:27:38.316Z Has data issue: false hasContentIssue false
  • English
  • Français

In situ Generation of Ruthenium Tetroxide and Osmium Tetroxide for the Physical Sciences and Their Reaction Indicators

Published online by Cambridge University Press:  14 March 2018

Paul Beauregard
Paul Beauregard*
Affiliation:
Chemist and Electron Microscopist
*
[email protected]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Recently, there was a suggestion on the MSA listserver about the use of osmium tetroxide (OsO4 and how to handle it. One suggestion was that ampoules be scored, placed in a glass jar, and the ampoule smashed to release the contents. This seemed like a very unsafe way to use osmium tetroxide or ruthenium tetroxide. The purpose of this article is to suggest a way to generate smaller amounts of these compounds in a safer manner than smashing ampoules and wondering about what to do with the unused portion after staining or storing. Another purpose is to discuss a new reaction indicator for mainly osmium tetroxide. The use of a reaction specific indicator was mandatory for judging the level or degree to which staining had proceeded in thin sections for the transmission electron microscope (TEM).

Type
Research Article
Information
Microscopy Today , Volume 10 , Issue 5 , September 2002 , pp. 20 - 23
Copyright
Copyright © Microscopy Society of America 2002

References

References:

1) Sawyer Linda, C., David, Grubb, Polymer Microscopy, Chapman and Hall, p 105 to 124, 1987.CrossRefGoogle Scholar
2) Albert, Cotton F., Wilkinson Geoffrey, Advanced Inorganic Chemistry A Comprehensive Text, second edition, Interscience, p 1005, 1966 Google Scholar