Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-02T18:59:13.030Z Has data issue: false hasContentIssue false
  • English
  • Français

Improved Cutting of One-Micron Plastic Sections using Qwick Glass Knives

Published online by Cambridge University Press:  14 March 2018

James T. McMahon  and
Joel Alsouss
James T. McMahon*
Affiliation:
Cleveland Clinic Foundation, Cleveland, Ohio
Joel Alsouss
Affiliation:
Cleveland Clinic Foundation, Cleveland, Ohio
*
[email protected]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

One-micron plastic "thick" sections, usually stained with toluidine blue, are indispensable light microscopic adjuvants for the selection and evaluation of tissue blocks prior to electron microscopy. Plastic thick sections may also be the only practical alternative to routine histologic sections when tissue specimens are limited or too small for processing in paraffin. In all cases, however, plastic sections provide superior light microscopic resolution compared to paraffin sections, but lack the compatibility with the wide variety of special stains that make paraffin sections so versatile.

Type
Research Article
Information
Microscopy Today , Volume 14 , Issue 3 , May 2006 , pp. 38 - 41
Copyright
Copyright © Microscopy Society of America 2006

References

1. Latta, H. and Hartmann, J. F. (1950). The use of a glass edge in sectioning for electron microscopy. Proc. Soc. Exptl. Biol. Med., 74,436.Google Scholar