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Dissecting Chromosomes, One at a Time!

Published online by Cambridge University Press:  14 March 2018

Stephen W. Carmichael*
Affiliation:
Mayo Clinic

Extract

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The atomic force microscope (AFM) is proving to be as much a tool as an imaging device. Recently, Stefan Thalhammer, Robert Stark, Stefan Müller, Johannes Wienberg and Wolfgang M. Heckl have added another useful technique to this repertoire. They demonstrated that the AFM can be used to cut out an identified section of DNA and then extract that section for amplification.

They began by flow sorting chromosomes, and then increased the specific amount of the target DNA with a “pre-set” in situ hybridization with chromosome-specific DMA to provide a specimen rich in chromosome 2. To identify the target area, selected chromosomes were examined with the AFM in the non-contact mode using stiff cantilevers.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 1998

References

2. Thalhammer, S., Stark, R.W., Müller, S., Wienberg, J., and Heckl, W.M., The atomic force microscope as a new microdissection tool for the generation of genetic probes, J. Structural Biol. 119. 232237, 1997 Google Scholar