Creating Pseudocolor Images using ImageJ

Extract

It is common for microscopists to examine fluorescent samples that have been exposed to a mixed set of fluorophores with specific staining properties. Images are often captured from these samples as a set, using different filter sets to isolate specific elements of the sample. Sometimes the images are captured in gray scale, and in other cases, they are captured with color cameras. In either case, one would often like to combine the images to create a single image that presents the information from all fluorophores at once. This kind of display is particularly useful for determining the relative locations of different components.