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X-Ray Microscopy Projects In Aarhus

Published online by Cambridge University Press:  02 July 2020

R. Medenwaldt ,
J. Abraham ,
E. E.UggerhØj ,
F. Vollrath  and
E. SØgaard
R. Medenwaldt
Affiliation:
Institute for Storage Ring Facilities, University of Aarhus, Denmark
J. Abraham
Affiliation:
Institute for Storage Ring Facilities, University of Aarhus, Denmark
E. E.UggerhØj
Affiliation:
Institute for Storage Ring Facilities, University of Aarhus, Denmark
F. Vollrath
Affiliation:
Department of Zoology, University of Aarhus, Denmark
E. SØgaard
Affiliation:
Department of Chemistry, University of Aalborg, Esbjerg, Denmark
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Extract

X-ray microscopy with soft X-rays is well suited for investigations of aqueous samples of some microns thickness when the resolution is required to be better than in visible light microscopy an not better than 30 nm. Sample preparation is as simple as for light microscopy, i.e. no fixation or metal coating is needed.

In Aarhus, an X-ray microscope is used for investigations in fields as biology, medicine and soil sciences. A ray diagram of the Aarhus X-ray microscope is shown in Fig.l. Synchrotron radiation at a wavelength of 2.4 nm from the Aarhus Storage Ring is focused by a condenser zone plate onto an object. Another zone plate as an objective behind the object forms the image on a CCD camera. Objects are located under atmospheric pressure. For dry samples, almost any kind of holder can be mounted in the microscope. Wet samples are placed between thin silicon foils in a sealed chamber. With typical liquid layer thickness of 5-15 µm, samples can be kept in the chamber for many hours without drying out.

Type
Quantitative Biological and Materials Microanalysis by Electrons and X-Rays
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 907 - 908
Copyright
Copyright © Microscopy Society of America 1997

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References

The condenser has been fabricated by G.Schmahl’s group at the Institute for X-ray physics, University of GÖttingen, and the objective zone plate by S.Spector from SUNY at St.Brook.Google Scholar