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What X-Ray Microanalysis Can Tell Us About The Regulation of Intracellular Calcium

Published online by Cambridge University Press:  02 July 2020

S.B. Andrews ,
N.B. Pivovarova ,
L.D. Pozzo-Miller  and
R.D. Leapman
S.B. Andrews
Affiliation:
Laboratory of Neurobiology, NINDS, Bethesda, MD, 20892-4062
N.B. Pivovarova
Affiliation:
Laboratory of Neurobiology, NINDS, Bethesda, MD, 20892-4062
L.D. Pozzo-Miller
Affiliation:
Laboratory of Neurobiology, NINDS, Bethesda, MD, 20892-4062
R.D. Leapman
Affiliation:
Biomédical Engineering 3x0026 Instrumentation Program, NCRR, National Institutes of Health, Bethesda, MD, 20892-4062.
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Extract

Many cell functions, including such critical processes as secretion, neuronal communication, muscle contraction, and even gene expression, are regulated by spatial and temporal changes in the intracellular concentration of Ca2+ ions. The high spatial resolution and analytical sensitivity of energy dispersive x-ray (EDX) microanalysis are ideally suited to characterizing biologically relevant changes in total concentration of cellular Ca. However, EDX results have not yet had a wide impact in the field of Ca regulation, with most laboratories preferring to focus on optically measured changes in free Ca concentrations as the important determinant of biological activity. This paper advocates a more positive perspective for the role of EDX microanalysis in studies of Ca regulation.

It is well known that the large majority of cellular Ca is in the bound form, with typical bound/free ratios on the order of l03-104 5. However, the most dramatic changes during Ca2+ -dependent cell activation occur as transients in the free Ca2+ pool.

Type
Quantitative Biological and Materials Microanalysis by Electrons and X-Rays
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 915 - 916
Copyright
Copyright © Microscopy Society of America 1997

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