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Ultrastructural Studies of Nipah Virus, A Newly Emergent Paramyxovirus, Using Thin Section, Negative Stain, Immunogold, and in Situ Hybridization Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

Cynthia S. Goldsmith ,
Toni Whistler ,
Pierre E. Rollin ,
Kaw Bing Chua ,
William Bellini ,
Paul Rota ,
K. T. Wong ,
Peter Daszak ,
Thomas G. Ksiazek  and
Sherif R. Zaki
Cynthia S. Goldsmith
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Toni Whistler
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Pierre E. Rollin
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Kaw Bing Chua
Affiliation:
University of Malaysia, Kuala Lumpur, Malaysia
William Bellini
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Paul Rota
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
K. T. Wong
Affiliation:
University of Malaysia, Kuala Lumpur, Malaysia
Peter Daszak
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Thomas G. Ksiazek
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
Sherif R. Zaki
Affiliation:
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia30333
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Extract

An increase in cases of acute febrile encephalitis occurred in Western Malaysia between September 1998 and May 1999, and a similar illness was reported in Singapore in March 1999. Most cases occurred in males who had been exposed to pigs, or among abattoir workers, and at least 100 deaths were reported. The illness was characterized by fever and headache, followed by drowsiness and disorientation; patients with severe cases developed seizures and coma within 24 to 48 hours. Concurrently, there were also illnesses and deaths among pigs in the same region, although the symptoms predominantly involved the respiratory system, and only a few pigs had signs of neurologic disease. From a cerebral spinal fluid specimen from a human patient, Dr. Chua and colleagues were successful in isolating a virus that was morphologically identified as belonging to the family Paramyxoviridae, and is now known as Nipah virus.

Type
Microorganisms: The Good, The Bad, The Unusual
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 644 - 645
Copyright
Copyright © Microscopy Society of America

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References

References:

1.CDC, MMWR 48(1999)265.Google Scholar
2.Chua, KB, et al., Lancet 354(1999)1257.CrossRefGoogle Scholar
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