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Ultrastructural Localization of CD34 in Soft Tissue Tumors

Published online by Cambridge University Press:  02 July 2020

J. M. Minda MS ,
T. J. Lawton MD  and
M. van de Rijn MD PhD.
J. M. Minda MS
Affiliation:
Anatomic Pathology Division, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pa.
T. J. Lawton MD
Affiliation:
Anatomic Pathology Division, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pa.
M. van de Rijn MD PhD.
Affiliation:
Anatomic Pathology Division, Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pa.
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Extract

The CD34 antigen is a single chain, heavily glycosylated transmembrane protein originally identified in hematopoietic stem cells. Subsequently it was found to be expressed in cells of normal endothelium and a variety of soft tissue tumors, including: epithelioid sarcoma, solitary fibrous tumor,’ gastrointestinal stromal tumor and dermatofibrosarcoma protuberans. Using paraffin immunohistochemistry, membranous and cytoplasmic staining for CD34 can be seen in these lesions, with most showing a combination of both. The findings of cytoplasmic staining is unexpected, as CD34 is a transmembrane cell surface protein. We analyzed the ultrastructural localization of CD34 on two soft tissue tumors with membranous and combined membranous and cytoplasmic staining.

Biopsy specimens were fixed with a mixture of 4% paraformaldehyde and 1% glutaraldehyde in 0.1M sodium cacodylate buffer, pH = 7.4 for 1-2 hours, and embedded in Poly/Bed 812 with osmium tetroxide post-fixation, or in LR White without osmication. The immunogold method was performed on ultrathin sections placed on Nickel grids,

Type
Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1084 - 1085
Copyright
Copyright © Microscopy Society of America

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