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Ultrastructural and Cytochemical Analyses of the Expression of the Thiobacillus Carboxysome Operon in Escherichia Coli

Published online by Cambridge University Press:  02 July 2020

H.C. Aldrich ,
S. Elvington ,
HE. Machines ,
R. Szabady ,
K. Feder ,
L. McDowell  and
J.M. Shively
H.C. Aldrich
Affiliation:
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611-0700
S. Elvington
Affiliation:
Department of Biological Sciences, Clemson University, Clemson, SC, 29634-1903
HE. Machines
Affiliation:
Department of Biological Sciences, Clemson University, Clemson, SC, 29634-1903
R. Szabady
Affiliation:
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611-0700
K. Feder
Affiliation:
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611-0700
L. McDowell
Affiliation:
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611-0700
J.M. Shively
Affiliation:
Department of Biological Sciences, Clemson University, Clemson, SC, 29634-1903
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Abstract

The cytoplasm of the bacterium Thiobacillus neapolitanus contains 117 nm diameter polyhedral inclusions, “carboxysomes” (Fig. 1) that contain ribulose-1,5- bisphosphate carboxylase/oxygenase (RuBisCO). Surrounding the polyhedron are nonmembranous proteinaceous plates devoid of lipid. The carboxysomes are composed of at least 8 major peptides, all coded within the same operon. Six (CsoSIA, CsoSIB, CsoSIC, CsoS2A, CsoS2B, and CsoS3) make up the shell, and two are the large (CbbL) and small subunits (CbbS) of RuBisCO. Using immunogold labeling on ultrathin sections, peptides CsoS2A, CsoS2B, and CsoS3 have been localized to the shell. Since the original characterization of the csoSl gene, we have also immunolocalized the CsoSl peptide to the shell.

As part of our initial efforts to understand how these components are assembled into the symmetrical, functional entity, the carboxysome operon from T. neapolitanus was cloned into the pET-21a(+) plasmid, an expression vector that codes for resistance to ampicillin.

Type
Microbiology
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 740 - 741
Copyright
Copyright © Microscopy Society of America 2001

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References

1.Shively, J.M., et al., Science 182:584–586 (1973).CrossRefGoogle Scholar
2.Shively, J.M., et al., in Lidstrom, M.E. and Tabita, F.R. (eds.) Microbial growth on CI compounds, Dordrecht: Kluwer Academic Publishers (1996) 56–63.CrossRefGoogle Scholar
3.Baker, S.H., et al., Arch. Microbiol. 172:233–239 (1999)CrossRefGoogle Scholar
4.S.H., Baker, et al., Arch. Microbiol. 173:278–283 (2000).Google Scholar
5.R.S., English et al., Mol. Microbiol. 12:647–654 (1994).Google Scholar