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Two-Photon Microscopy as a Useful Tool for Elucidating the Mode of Action of Novel Potential Cancer Therapeutics.

Published online by Cambridge University Press:  02 July 2020

C. S. Navara  and
F. M. Uckun
C. S. Navara
Affiliation:
Departments of Experimental Oncology, St. Paul, MN55113
F. M. Uckun
Affiliation:
Drug Discovery Program Hughes Institute, St. Paul, MN55113
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Extract

Multiphoton microscopy allows us to follow specific cellular structures (ie. nuclei, mitochondria, and the plasma membrane) in a high resolution three dimensional and time resolved fashion. We have used multiphoton confocal microscopy to evaluate the cellular effects of potential cancer therapeutics. Cancer cells were plated onto coverslips and labelled with either the vital DNA dye Hoechst to image the nuclei and chromosomes or the lipid dye DiA to label the plasma membrane. Both of these dyes can be imaged with several sections without affecting cell viability as assessed by cell motility and the ability to undergo mitosis and cytokinesis.

We have used this technology to show that one compound (DDE 131) rapidly induces in cancer cell lines the hallmarks of apoptosis; DNA hypercondensation, nuclear fragmentation and rapid membrane blebbing. In a leukemic cell line these changes take place within 20 minutes of treatment.

Type
Multi Photon Excitation Microscopy: The Next Generation
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1064 - 1065
Copyright
Copyright © Microscopy Society of America

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