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Two-Photon Imaging of Cellular Activities in Oxygen Sensing Tissues

Published online by Cambridge University Press:  06 November 2008

Christoph Wotzlaw ,
Utta Berchner-Pfannschmidt ,
Joachim Fandrey  and
Helmut Acker
Christoph Wotzlaw
Affiliation:
Institut für Physiologie, Universität Duisburg-Essen, Hufelandstraße 55, D-45122 Essen, Germany
Utta Berchner-Pfannschmidt
Affiliation:
Institut für Physiologie, Universität Duisburg-Essen, Hufelandstraße 55, D-45122 Essen, Germany
Joachim Fandrey
Affiliation:
Institut für Physiologie, Universität Duisburg-Essen, Hufelandstraße 55, D-45122 Essen, Germany
Helmut Acker*
Affiliation:
Institut für Physiologie, Universität Duisburg-Essen, Hufelandstraße 55, D-45122 Essen, Germany
*
Corresponding author. E-mail: [email protected]
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Abstract

The cellular oxygen sensing system of the body ensures appropriate adaptation of cellular functions toward hypoxia by regulating gene expression and ion channel activity. Two-photon laser microscopy is an ideal tool to study and prove the relevance of the molecular mechanisms within oxygen sensing pathways on the cellular and complex tissue or organ level. Images of hypoxia inducible factor 1 (HIF-1) subunit nuclear mobility and protein-protein interaction in living cells, of hypoxia-induced changes in membrane potential and intracellular calcium of live ex vivo carotid bodies as well as of rat kidney proximal tubulus function in vivo, will be shown.

Keywords

oxygen sensingHIFmembrane potential(Ca++)iU2OS cellcarotid bodykidneyFRETFRAP3D imaging
Type
Multiphoton Microscopy–Special Section
Information
Microscopy and Microanalysis , Volume 14 , Issue 6 , December 2008 , pp. 519 - 525
Copyright
Copyright © Microscopy Society of America 2008

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References

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