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Two Microtubule Organizing Centers are Anchored in the Cortex Prior to Mammalian Oocyte Maturation

Published online by Cambridge University Press:  02 July 2020

Patricia G. Calarco
Patricia G. Calarco*
Affiliation:
Dept. of Anatomy, University of California, San Francisco, CA, 94143
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Extract

Oocyte meiotic maturation involves complex processes of nuclear breakdown, spindle formation, and organelle movement necessary for successful fertilization and early development. Yet these take place in the mouse without the aid of centrioles; instead the spindle is organized by a number of microtubule organizing centers (MTOC).Multiple MTOC are seen by fluorescence microscopy to migrate from the cortex to the nucleus (germinal vesicle; GV) during the first 30 min of maturation. However, EM has not revealed the expected ultrastructure of MTOC known from previous studies. New information from the present study sheds light on MTOC number, location and ultrastructure prior to and during very early maturation.

Maturation occurs in vitro when oocytes are released from ovarian follicles into culture, but is blocked by dibutyrl cAMP or IBMX (3-isobutyl-l-methyl xanthine; Sigma). MTOC were studied after 0-2 h in culture medium; IBMX medium (0.2 mM); an inhibitor of MT (nocodazole, lμM); or MF (cytochalasin, 5 μg/ml) by fluorescent confocal microscopy (CM; Biorad 600) or standard EM.

Type
Imaging Cells and Organelles
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 237 - 238
Copyright
Copyright © Microscopy Society of America 1997

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References

1 Calarco, P. et al., 1972, J. Cell Sci. 10:369–385.Google Scholar

2 Calarco, P. et al., 1983, Cell 35:621–629.CrossRefGoogle Scholar

3 Van Blerkom, J., 1991, Proc. Natl. Acad. Sei. USA 88:5031–5035.Google Scholar

4 Calarco, P., 1955, Develop. Genetics 16:36–43.CrossRefGoogle Scholar