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A Transmission Electron Microscopy Study and Viability Evaluation of the Effect of 2450 MHz Microwaves on Candida albicans.

Published online by Cambridge University Press:  02 July 2020

C. Melgoza ,
J. Sepúlveda ,
M. Moreno  and
M. A. Rodríguez
C. Melgoza
Affiliation:
Department of Microbiology, Universidad Autónoma de Nuevo León, Facultad de Medicina, A.P. 1563, Monterrey, N.L., México.
J. Sepúlveda
Affiliation:
Dept.of Histology, andUniversidad Autónoma de Nuevo León, Facultad de Medicina, A.P. 1563, Monterrey, N.L., México.
M. Moreno
Affiliation:
Dept. of Pharmacology. Universidad Autónoma de Nuevo León, Facultad de Medicina, A.P. 1563, Monterrey, N.L., México.
M. A. Rodríguez
Affiliation:
Department of Microbiology, Universidad Autónoma de Nuevo León, Facultad de Medicina, A.P. 1563, Monterrey, N.L., México.
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Extract

Microwaves of 2450 MHz have been used as a heating procedure in food technology, tissue processing for histological studies (1), in medicine (2) and as a sterilization agent for medical devices (3). Experiments were carried out exposing yeast cells of Candida albicans suspended in growth media to different times of radiation at 750 watts in a commercial microwave oven. Batches of 5 tubes containing the cells suspension were radiated from 15 seconds to 3 minutes and colony counts were done after exposure. Viability ceased totally after one minute radiation. Samples were obtained from unexposed controls and tubes radiated for 15, 30, 45 and 60 seconds, centrifuged and the pellets fixed in 1.5% potasium permanganate. After embbeding in Epon, thin sections were obtained and stained with uranyl acetate. Micrographs were taken with a Zeiss EM-109 electron microscope. Cells showed alteration of internal membranes at early times, and later changes indicative of cytoplasmic coagulation and organelle disorganization were seen. At 15 seconds mitochondrial matrix showed alterations followed by damage of its membranes.

Type
Non-Vertebrate Biology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 117 - 118
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Boon, M.E. and Kok, L.P., Microwave cookbook of pathology, ed. Coulomb press, Leyden Netherlands, (1988).Google Scholar
2.Thuery, J., Microwaves: industrial, scientific and medical aplications, Artech house, Boston- London (1992).Google Scholar
3.Latimer, J. M. and Matsen, J.M., Microwave oven irradiation as a method for bacterial decontamination in a clinical microbioloy laboratory, J. Clin. Microbiol. Vol. 17, 340, (1993).Google Scholar