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Transmission Electron Microscopy of Rat Kupffer Cell Activation After Cisplatin Treatment: an in Vivo Study

Published online by Cambridge University Press:  02 July 2020

D.J. Telgenhoff  and
S.K. Aggarwal
D.J. Telgenhoff
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI48824-1115
S.K. Aggarwal
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI48824-1115
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Extract

Cisplatin (cis-diamminedichloroplatinum II) is a broad spectrum anti-cancer agent. Its main limitations are its severe toxic side effects. Cisplatin’s major mechanisms of action include inhibition of DNA synthesis by interstrand and intrastrand crosslinking, inhibition of cytokinesis, and macrophage activation. Cisplatin acts through the activation of the immune system by inducing macrophages to form cytoplasmic extensions which seek out and phagocytose tumor cells. More recently, cisplatin has been shown to activate Kupffer cells (macrophages in the liver) by increasing their number and cytoplasmic extensions. Electron microscopic analysis of cisplatin treated rat liver in vivo was performed to examine these extensions in greater detail and relate these to other changes in the liver cells.

Wistar rats (100-130 g) were given intraperitoneal injections of cisplatin (9 mg/kg in 0.9% sodium chloride) over a five day period. Controls were treated with equal amounts of the vehicle of injection. After 9 days the rats were sacrificed. Segments of liver were fixed in 2% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3).

Type
Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 83 - 84
Copyright
Copyright © Microscopy Society of America 1997

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