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Transmission Electron Microscopy of Lipid Vesicles for Drug Delivery: Comparison between Positive and Negative Staining

Published online by Cambridge University Press:  22 June 2010

Valentina Bello ,
Giovanni Mattei ,
Paolo Mazzoldi ,
Nicoletta Vivenza ,
Paolo Gasco ,
Jean Marc Idee ,
Caroline Robic  and
Elisabetta Borsella
Valentina Bello*
Affiliation:
Department of Physics, University of Padova, via Marzolo 8, I-35131 Padova, Italy
Giovanni Mattei
Affiliation:
Department of Physics, University of Padova, via Marzolo 8, I-35131 Padova, Italy
Paolo Mazzoldi
Affiliation:
Department of Physics, University of Padova, via Marzolo 8, I-35131 Padova, Italy
Nicoletta Vivenza
Affiliation:
Nanovector S.r.l., via Livorno, 60, I-10144 Torino, Italy
Paolo Gasco
Affiliation:
Nanovector S.r.l., via Livorno, 60, I-10144 Torino, Italy
Jean Marc Idee
Affiliation:
Guerbet, 15 rue des Vanesses, Zone Paris Nord I, 93420 Villepinte (Paris), France
Caroline Robic
Affiliation:
Guerbet, 15 rue des Vanesses, Zone Paris Nord I, 93420 Villepinte (Paris), France
Elisabetta Borsella
Affiliation:
ENEA, Dept. FIM, via E. Fermi 45, I-00044 Frascati (Rome), Italy
*
Corresponding author. E-mail: [email protected]
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Abstract

Lipid-containing nanostructures, in the form of solid lipid nanoparticles or iron oxide nanoparticles (NPs) coated with a lipid shell, were used as case studies for assessing and optimizing staining for transmission electron microscopy structural and compositional characterization. These systems are of paramount importance as drug delivery systems or as bio-compatible contrast agents. In particular, we have treated the systems with a negative (phospshotungstic acid) or with a positive (osmium tetroxide) staining agent. For iron-oxide NPs coated with the lipid shell, negative staining was more efficient with respect to the positive one. Nevertheless, in particular cases the combination of the two staining procedures provided more complete morphological and compositional characterization of the particles.

Keywords

lipid nanoparticlesUSPIOstaining processTEM
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 4 , August 2010 , pp. 456 - 461
Copyright
Copyright © Microscopy Society of America 2010

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References

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