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Transmission Electron Microscopy, Fluorescence Microscopy, and Confocal Raman Microscopic Analysis of Ultrastructural and Compositional Heterogeneity of Cornus alba L. Wood Cell Wall

Published online by Cambridge University Press:  04 February 2013

Jianfeng Ma ,
Zhe Ji ,
Xia Zhou ,
Zhiheng Zhang  and
Feng Xu
Jianfeng Ma
Affiliation:
Institute of Biomass Chemistry and Technology, Beijing Forestry University, 100083 Beijing, China
Zhe Ji
Affiliation:
Institute of Biomass Chemistry and Technology, Beijing Forestry University, 100083 Beijing, China
Xia Zhou
Affiliation:
Institute of Biomass Chemistry and Technology, Beijing Forestry University, 100083 Beijing, China
Zhiheng Zhang
Affiliation:
Institute of Biomass Chemistry and Technology, Beijing Forestry University, 100083 Beijing, China
Feng Xu*
Affiliation:
Institute of Biomass Chemistry and Technology, Beijing Forestry University, 100083 Beijing, China College of Light Industry and Textiles, Qiqihar University, 161006 Qiqihar, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

Transmission electron microscopy (TEM), fluorescence microscopy, and confocal Raman microscopy can be used to characterize ultrastructural and compositional heterogeneity of plant cell walls. In this study, TEM observations revealed the ultrastructural characterization of Cornus alba L. fiber, vessel, axial parenchyma, ray parenchyma, and pit membrane between cells, notably with the ray parenchyma consisting of two well-defined layers. Fluorescence microscopy evidenced that cell corner middle lamella was more lignified than adjacent compound middle lamella and secondary wall with variation in lignification level from cell to cell. In situ Raman images showed that the inhomogeneity in cell wall components (cellulose and lignin) among different cells and within morphologically distinct cell wall layers. As the significant precursors of lignin biosynthesis, the pattern of coniferyl alcohol and aldehyde (joint abbreviation Lignin-CAA for both structures) distribution in fiber cell wall was also identified by Raman images, with higher concentration occurring in the fiber secondary wall where there was the highest cellulose concentration. Moreover, noteworthy was the observation that higher concentration of lignin and very minor amounts of cellulose were visualized in the pit membrane areas. These complementary microanalytical methods provide more accurate and complete information with regard to ultrastructural and compositional characterization of plant cell walls.

Keywords

Cornus alba L.TEMconfocal Raman microscopyultrastructurecomponents distribution
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 243 - 253
Copyright
Copyright © Microscopy Society of America 2013

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Footnotes

†

Current address: Institute of Material Science and Technology, Beijing Forestry University, Beijing, 100083, China

References

Agarwal, U.P. (1999). An overview of Raman spectroscopy as applied to lignocellulosic materials. In Advances in Lignocellulosics Characterization, Argyropoulos, D.S. (Ed.), pp. 201–225. Atlanta, GA: TAPPI Press.Google Scholar
Agarwal, U.P. (2006). Raman imaging to investigate ultrastructure and composition of plant cell walls: Distribution of lignin and cellulose in black spruce wood (Picea mariana). Planta 224(5), 1141–1153.CrossRefGoogle ScholarPubMed
Agarwal, U.P. & Atalla, R.H. (1986). In-situ Raman microprobe studies of plant cell walls: Macromolecular organization and compositional variability in the secondary wall of Picea mariana (Mill.) B.S.P. Planta 169(3), 325–332.Google Scholar
Agarwal, U.P., McSweeny, J.D. & Ralph, S.A. (2011). FT-Raman investigation of milled-wood lignins: Softwood, hardwood, and chemically modified black spruce lignins. J Wood Chem Technol 31(4), 324–344.Google Scholar
Agarwal, U.P. & Ralph, S.A. (1997). FT-Raman spectroscopy of wood: Identifying contributions of lignin and carbohydrate polymers in the spectrum of black spruce. Appl Spectrosc 51(11), 1648–1655.Google Scholar
Boerjan, W., Ralph, J. & Baucher, M. (2003). Lignin biosynthesis. Annu Rev Plant Biol 54(1), 519–546.Google Scholar
Chaffey, N., Barlow, P. & Sundberg, B. (2002). Understanding the role of the cytoskeleton in wood formation in angiosperm trees: Hybrid aspen (Populus tremula x P. tremuloides) as the model species. Tree Physiol 22(4), 239–249.CrossRefGoogle Scholar
Cosgrove, D.J. (2005). Growth of the plant cell wall. Nat Rev Mol Cell Bio 6(11), 850–861.Google Scholar
Daniel, G., Nilsson, T. & Pettersson, B. (1991). Poorly and non-lignified regions in the middle lamella cell corners of birch (Betula verrucosa) and other wood species. IAWA Bull 12, 70–83.CrossRefGoogle Scholar
Donaldson, L., Hague, J. & Snell, R. (2001). Lignin distribution in coppice poplar, linseed and wheat straw. Holzforschung 55(4), 379–385.Google Scholar
Donaldson, L.A. (2001). Lignification and lignin topochemistry: An ultrastructural view. Phytochemistry 57(6), 859–873.Google Scholar
Donaldson, L.A. (2008). Microfibril angle: Measurement, variation and relationships. IAWA J 29(4), 345–386.Google Scholar
Donaldson, L.A. & Knox, J.P. (2012). Localization of cell wall polysaccharides in normal and compression wood of radiata pine: Relationships with lignification and microfibril orientation. Plant Physiol 158(2), 642–653.Google Scholar
Donaldson, L.A. & Lausberg, M.J.F. (1998). Comparison of conventional transmitted light and confocal microscopy for measuring wood cell dimensions by image analysis. IAWA J 19(3), 321–336.Google Scholar
Donaldson, L.A. & Ryan, K.G. (1987). A comparison of relative lignin concentration as determined by interference microscopy and bromination/EDXA. Wood Sci Technol 21(4), 303–309.Google Scholar
Donaldson, L.A., Singh, A.P., Yoshinaga, A. & Takabe, K. (1999). Lignin distribution in mild compression wood of Pinus radiata . Can J Bot 77(1), 41–50.Google Scholar
Feild, T.S., Lee, D.W. & Holbrook, N.M. (2001). Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood. Plant Physiol 127(2), 566–574.Google Scholar
Fengel, D. & Wegener, G. (1984). Wood: Chemistry, Ultrastructure, Reactions. Walter de Gruyter, Berlin: Walter de Gruyter Press.Google Scholar
Fromm, J., Rockel, B., Lautner, S., Windeisen, E. & Wanner, G. (2003). Lignin distribution in wood cell walls determined by TEM and backscattered SEM techniques. J Struct Biol 143(1), 77–84.Google Scholar
Gierlinger, N. & Schwanninger, M. (2006). Chemical imaging of poplar wood cell walls by confocal Raman microscopy. Plant Physiol 140(4), 1246–1254.Google Scholar
Gierlinger, N. & Schwanninger, M. (2007). The potential of Raman microscopy and Raman imaging in plant research. Spectroscopy 21(2), 69–89.Google Scholar
Jansen, S., Choat, B. & Pletsers, A. (2009). Morphological variation of intervessel pit membranes and implications to xylem function in angiosperms. Am J Bot 96(2), 409–419.Google Scholar
Kutscha, N.P. & McOrmond, R.R. (1972). The suitability of using fluorescence microscopy for studying lignification in balsam fir. Technical Bulletin 62. Life Sciences and Agriculture Experiment Station, University of Maine, Orono, Maine. Google Scholar
Murphy, R.J. & Alvin, K.L. (1992). Variation in fiber wall structure in bamboo. IAWA Bull 13(4), 403–410.Google Scholar
Parameswaran, N. & Liese, W. (1976). On the fine structure of bamboo fibers. Wood Sci Technol 10(4), 231–246.Google Scholar
Parham, R.A. & Côté, W.A. (1971). Distribution of lignin in normal and compression wood of Pinus taeda L. Wood Sci Technol 5(1), 49–62.Google Scholar
Ralph, J., Brunow, G. & Boerjan, W. (2007). Lignins. In Encyclopedia of Life Sciences. Chichester, UK: Wiley.Google Scholar
Saka, S., Whiting, P., Fukazawa, K. & Goring, D. (1982). Comparative studies on lignin distribution by UV microscopy and bromination combined with EDXA. Wood Sci Technol 16(4), 269–277.Google Scholar
Schmid, R. & Machado, R.D. (1968). Pit membranes in hardwoods—Fine structure and development. Protoplasma 66(1-2), 185–204.Google Scholar
Singh, A., Daniel, G. & Nilsson, T. (2002). Ultrastructure of the S2 layer in relation to lignin distribution in Pinus radiata tracheids. J Wood Sci 48(2), 95–98.Google Scholar
Singh, A.P., Kim, Y.S., Chung, G.C., Park, B.D. & Wong, A.H.H. (2003). TEM examination of surface characteristics of rubberwood (Hevea brasiliensis) HTMP fibers. Holzforschung 57(6), 579–584.Google Scholar
Takayama, M., Johjima, T., Yamanaka, T., Wariishi, H. & Tanaka, H. (1997). Fourier transform Raman assignment of guaiacyl and syringyl marker bands for lignin determination. Spectrochim Acta A 53(10), 1621–1628.Google Scholar
Timell, T.E. (1986). Chemical properties of compression wood. In Compression Wood in Gymnosperms, vol. 1, pp. 289–408. Berlin: Springer Verlag.Google Scholar
Tirumalai, V., Agarwal, U. & Obst, J. (1996). Heterogeneity of lignin concentration in cell corner middle lamella of white birch and black spruce. Wood Sci Technol 30(2), 99–104.CrossRefGoogle Scholar
Westermark, U., Lidbrandt, O. & Eriksson, I. (1988). Lignin distribution in spruce (Picea abies) determined by mercurization with SEM-EDXA technique. Wood Sci Technol 22(3), 243–250.Google Scholar
Wi, S.G., Singh, A.P., Lee, K.H. & Kim, Y.S. (2005). The pattern of distribution of pectin, peroxidase and lignin in the middle lamella of secondary xylem fibers in alfalfa (Medicago sativa). Ann Bot 95(5), 863–868.Google Scholar
Wiley, J.H. & Atalla, R.H. (1987). Band assignments in the raman spectra of celluloses. Carbohydr Res 160(220), 113–129.Google Scholar
Xu, F., Sun, R.C., Lu, Q. & Jones, G. (2006). Comparative study of anatomy and lignin distribution in normal and tension wood of Salix gordejecii . Wood Sci Technol 40(5), 358–370.Google Scholar