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Transfer Cell Development And Immunolocalization Of A Senescence Associated Gene (Sag14) In Senescing Arabidopsis Thaliana Leaves.

Published online by Cambridge University Press:  02 July 2020

W. A. Russin ,
L. M. Weaver  and
R. M. Amasino
W. A. Russin
Affiliation:
Department of Plant Pathology, University of Wisconsin, Madison, WI53706
L. M. Weaver
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI53706
R. M. Amasino
Affiliation:
Department of Biochemistry, University of Wisconsin, Madison, WI53706
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Extract

Leaf senescence is an orderly, active process in which nutrients in a leaf are reclaimed and mobilized to other parts of the plant. It is considered to be the final stage in leaf development. During senescence leaves undergo highly coordinated changes in cell structure, metabolism, and gene expression. For example, chloroplasts undergo obvious structural modifications during leaf senescence. Regarding gene expression, genes with steady-state mRNA levels that increase during senescence are often referred to as senescence-associated genes, or SAGs.

In order to study ultrastructural changes that occur during senescence, portions of fully expanded, mid-senescent, and fully senescent Arabidopsis thaliana leaves were chemically fixed using vacuum-accelerated microwave processing. Thin sections were observed for ultrastructural changes such as development of transfer cells that are characterized by the presence of cell wall ingrowths. Transfer cell development is correlated with an increased capacity of tissues to take up materials, related partly to an increase in plasma membrane surface area.

Type
Application Of Correlative Microscopy To Studies Of Plant Cell Function
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1252 - 1253
Copyright
Copyright © Microscopy Society of America

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References

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