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Three-dimensional Analysis of Laser Scanning Confocal Microscope Sections Reveals an Array of Microtubules in the Cleavage Furrow of Sea Urchin Eggs

Published online by Cambridge University Press:  02 February 2002

K. Larkin  and
M.V. Danilchik
K. Larkin*
Affiliation:
Department of Cell and Developmental Biology, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201 Department of Biological Structure and Function, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201
M.V. Danilchik
Affiliation:
Department of Cell and Developmental Biology, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201 Department of Biological Structure and Function, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201
*
*Corresponding author, at Oregon Regional Primate Research Center, 505 N.W. 185th Avenue, Beaverton, OR 97006.
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Abstract

Our recent observation that microtubules (MTs) are required for completion of division (abscission) led us to analyze MT organization during cytokinesis. Although many studies of MTs in sea urchin eggs have been done, computer-aided analysis of optical sections described herein reveals a new MT assemblage, which we call furrow MTs. This assemblage comprises bundles of MTs that lie in the cleavage furrow. Furrow MTs become apparent when the furrow has progressed approximately one-third of the way through the egg and persist to abscission. Furrow MTs are 8–24-µm long and arc across the base of the cleavage furrow. Acetylated tubulin is localized primarily in the furrow suggesting a distinct MT population. Three-dimensional analysis of optical sections suggests that furrow MTs are spatially distinct from midbody and astral MTs.

Keywords

confocalthree-dimensional reconstructionsea urchincell divisionmicrotubuleabscission
Type
Research Article
Information
Microscopy and Microanalysis , Volume 7 , Issue 3 , May 2001 , pp. 265 - 275
Copyright
Copyright © Microscopy Society of America 2001

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