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Three Dimensional Molecular Scale Imaging of Plasma Proteins Under Aqueous Conditions

Published online by Cambridge University Press:  02 July 2020

Roger E. Marchant ,
Nolan B. Holland  and
Christopher A. Siedlecki
Roger E. Marchant
Affiliation:
Department of Biomedical Engineering and Center for Cardiovascular Biomaterials, Case Western Reserve University, Cleveland, OH, 44106.
Nolan B. Holland
Affiliation:
Department of Biomedical Engineering and Center for Cardiovascular Biomaterials, Case Western Reserve University, Cleveland, OH, 44106.
Christopher A. Siedlecki
Affiliation:
Department of Biomedical Engineering and Center for Cardiovascular Biomaterials, Case Western Reserve University, Cleveland, OH, 44106.
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Extract

Atomic force microscopy (AFM) provides unique opportunities to study cell-surface and molecular scale interactions in three dimensions under aqueous conditions. In AFM, a small probe attached to cantilever (fig. 1) is used for interacting with a sample to obtain sensitive measurements of molecular structure, intermolecular forces (sub-nN) and interfacial properties. The data ouput can be presented in the form of a topographical image, force map or viscoelastic response (e.g., Figs. 2-4). Thus, AFM combines high resolution imaging with the ability to measure surface-dependent intermolecular forces and properties. AFM also is amenable for coupling with optical imaging methods including immunofluoresence and gold bead labeling.

This presentation will focus primarily on the use of AFM for molecular level imaging of plasma proteins, von Willebrand Factor (vWF) and fibrinogen. Both proteins play central roles in the regulation of hemostasis and thrombosis by participating in coagulation or by facilitating adhesion, spreading and aggregation of activated platelets.

Type
From Scanning Probe Microscopy to High Resolution Ultrasound: New Versions of the Vasculature
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 331 - 332
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Siedlecki, C.A. and Marchant, R.E., Biomaterials (in press).Google Scholar
2.Siedlecki, C.A., et al., Blood, 88 (1996) 2939.10.1182/blood.V88.8.2939.bloodjournal8882939CrossRefGoogle Scholar
3.Marchant, R.E., et al., Thrombosis and Haemostasis (in press).Google Scholar
4. The authors gratefully acknowledge use of the facilities of the Center for Cardiovascular Biomaterials, and financial support from NIH grant HL-40047, and the American Heart Association.Google Scholar