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Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology

Published online by Cambridge University Press:  19 November 2010

Zuzana Burdíková ,
Martin Čapek ,
Pavel Ostašov ,
Jiří Machač ,
Radek Pelc ,
Edward A.D. Mitchell  and
Lucie Kubínová
Zuzana Burdíková*
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-14220 Prague 4, Czech Republic Institute of Geology and Paleontology, Faculty of Science, Charles University, Albertov 6, CZ-12843 Prague 2, Czech Republic
Martin Čapek
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-14220 Prague 4, Czech Republic Faculty of Biomedical Engineering, Czech Technical University in Prague, nám. Sítná 3105, CZ-27201 Kladno, Czech Republic
Pavel Ostašov
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-14220 Prague 4, Czech Republic
Jiří Machač
Affiliation:
Institute of Botany, Academy of Sciences of the Czech Republic, v.v.i., Zámek 1, CZ-25243 Průhonice, Czech Republic
Radek Pelc
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-14220 Prague 4, Czech Republic
Edward A.D. Mitchell
Affiliation:
Laboratory of Soil Biology, University of Neuchâtel, Rue Emile-Argand 11, CH-2000 Neuchâtel, Switzerland
Lucie Kubínová
Affiliation:
Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, CZ-14220 Prague 4, Czech Republic
*
Corresponding author. E-mail: [email protected].
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Abstract

Testate amoebae (TA) are a group of free-living protozoa, important in ecology and paleoecology. Testate amoebae taxonomy is mainly based on the morphological features of the shell, as examined by means of light microscopy or (environmental) scanning electron microscopy (SEM/ESEM). We explored the potential applications of confocal laser scanning microscopy (CLSM), two photon excitation microscopy (TPEM), phase contrast, differential interference contrast (DIC Nomarski), and polarization microscopy to visualize TA shells and inner structures of living cells, which is not possible by SEM or environmental SEM. Images captured by CLSM and TPEM were utilized to create three-dimensional (3D) visualizations and to evaluate biovolume inside the shell by stereological methods, to assess the function of TA in ecosystems. This approach broadens the understanding of TA cell and shell morphology, and inner structures including organelles and endosymbionts, with potential implications in taxonomy and ecophysiology.

Keywords

testate amoebaeconfocal microscopyenvironmental scanning electron microscopytaxonomyecophysiology
Type
Fluorescence and Confocal Microscopies
Information
Microscopy and Microanalysis , Volume 16 , Issue 6 , December 2010 , pp. 735 - 746
Copyright
Copyright © Microscopy Society of America 2010

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