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A TEM and SEM Study of the Effects of Exogenous Calcium Oxalate Crystals on Renal Epithelial Cells

Published online by Cambridge University Press:  02 July 2020

K.L. Vaughn ,
R.L. Hackett ,
S.R. Kahn  and
P.N. Shevock-Scott
K.L. Vaughn
Affiliation:
Department of Interdisciplinary Center for Biotechnology Research Electron Microscopy Core, University of Florida, Gainesville, Fl32611
R.L. Hackett
Affiliation:
Department of Pathology College of Medicine, University of Florida, Gainesville, Fl. 32611
S.R. Kahn
Affiliation:
Department of Pathology College of Medicine, University of Florida, Gainesville, Fl. 32611
P.N. Shevock-Scott
Affiliation:
Department of Pathology College of Medicine, University of Florida, Gainesville, Fl. 32611
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Extract

When confluent monolayers of Madin-Darby Canine Kidney (MDCK) renal epithelial cells are exposed to calcium oxalate monohydrate (COM) crystals suspended in the nutrient media, crystals settle onto the cell surface. Here, they become entangled with the specialized membrane structures of cilia and microvilli (Figures 2). The cell membrane surface is structurally altered as patches of membrane devoid of microvilli are found adjacent to the crystal entrapped on the surface (Figure 4). Many COM crystals disappear from the cell surface and appear to be internalized. This phenomenon is seemingly common to all cultured renal epithelial cells. The uptake of crystals appear to increase with time and crystal concentration and stimulates a series of complex reactions. Morphologically, crystal aggregates are contained with intracellular vesicles which appear to be membrane lined (Figure 2,3). In addition, cells exocytose the internalized crystals and in MDCK cells, crystal-cytoplasm complexes can be observed leaving cells at the intercellular junctions.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 61 - 62
Copyright
Copyright © Microscopy Society of America 1997

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References

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