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Surface Morphological Changes Of Hela Cells Exposed To Tamoxifen And Taxol

Published online by Cambridge University Press:  02 July 2020

S.K. Majumdar  and
J. Valdellon
S.K. Majumdar
Affiliation:
Department of Biology, Lafayette College, Easton, PA18042
J. Valdellon
Affiliation:
Department of Biology, Lafayette College, Easton, PA18042
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Extract

Tamoxifen and taxol are two promising chemotherapeutic agents currently used in cancer treatment, but each has its own unique mechanism of action. Tamoxifen, known to inhibit the activity of estrogen on malignant mammary gland cells, was recently approved by the FDA for the treatment of breast cancer. Taxol, extracted from the Pacific Yew tree, Taxus brevifolia, is an antimitotic agent that binds specifically to the β-subunit, promoting the assembly and stabilization of tubulin polymers. Studies have shown that tamoxifen and taxol induce cytostatic and cytotoxic effects leading to the death of several cancer cell types in vitro.In this research, the surface ultrastructural effects of both tamoxifen and taxol were investigated in human cervical cancer cells (HeLa).

Exponentially growing cells were treated with 5, 10, 15, and 20 μg/ml of tamoxifen and 1 μg/ml of taxol (LC50) for 24, 48, 72, and 96 hours. For scanning electron microscopy (SEM), the cells were grown on coverslips, fixed in 2% glutaraldehyde followed by 1% osmium tetroxide.

Type
Biological Ultrastructure(Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1270 - 1271
Copyright
Copyright © Microscopy Society of America

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