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Structural Cell Biology: Preparing Specimens for Cryo-Electron Tomography Using Focused-Ion-Beam Milling

Published online by Cambridge University Press:  27 August 2014

Elizabeth Villa ,
Miroslava Schaffer ,
Ben Engel ,
Jürgen Plitzko  and
Wolfgang Baumeister
Elizabeth Villa
Affiliation:
Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, USA. Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
Miroslava Schaffer
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
Ben Engel
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.
Jürgen Plitzko
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany. Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
Wolfgang Baumeister
Affiliation:
Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 20 , Supplement S3: Proceedings of Microscopy & Microanalysis 2014 , August 2014 , pp. 1222 - 1223
Copyright
Copyright © Microscopy Society of America 2014 

References

[1] Rigort, A, et al, PNAS 109 (2012). p. 444.Google Scholar
[2] Villa, E, et al, Current Opinion in Structural Biology, 23 (2014). p. 77.Google Scholar
[3] Marko, M, et al, Nat Methods 4(3) (2007). p.215.Google Scholar
[4] Work was supported by the European Commission’s 7th Framework Programme grant agreement HEALTH-F4-2008-201648/PROSPECTS, the Deutsche Forschungsgemeinschaft Excellence Cluster CIPSM and SFB 1035 (to WB) and GRK 1721 (to EV), the Federal Ministry of Education and Research (BMBF), and from an inter-institutional research initiative of the Max Planck Society.Google Scholar