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Structural Analysis of Large-Scale Chromatin Organization

Published online by Cambridge University Press:  02 July 2020

A. S. Belmont ,
G. Li ,
G. Sudlow ,
T. Tumbar ,
Y. Strukov ,
S. Basu  and
A. Delaney
A. S. Belmont
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
G. Li
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
G. Sudlow
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
T. Tumbar
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
Y. Strukov
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
S. Basu
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
A. Delaney
Affiliation:
Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801
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Extract

Our laboratory is interested in understanding how 10 and 30 nm chromatin fibers fold to form interphase and mitotic chromosomes. Experimentally this has been a very difficult problem to investigate due to a number of technical difficulties. A common approach to this level of chromatin organization has been to use protein extraction conditions which experimentally “unravel” the native chromosome architecture. The difficulty with this approach is separating in vitro produced artifacts from remnants of in vivo structure.

As an alternative strategy we are focusing on changes in interphase chromosome structure during cell cycle progression and initiation of transcription or DNA replication, with the goal of identifying intermediates in the pathway of chromosome condensation or decondensation. A key element of this strategy is preserving chromosome structure as close as possible to its in vivo structure while using 3-dimensional light and electron microscopy reconstruction methods to “computationally” unravel native chromosome structure.

Type
Innovative Approaches to 3-D Structure/Function Determination for Cells and Organelles
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 215 - 216
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Belmont, A. S., Bruce, K., J. Cell Biol. (1994) 127, 28710.1083/jcb.127.2.287CrossRefGoogle Scholar
2.Robinett, C., Willhelm, C., Li, G., Belmont, A., Mol. Biol. Cell 5(suppl.), 3aGoogle Scholar
3.Robinett, C. C., Straight, A., Li, G., Willhelm, C., Sudlow, G., Murray, A., Belmont, A. S., J Cell Biol. (1996) 135, 168510.1083/jcb.135.6.1685CrossRefGoogle Scholar