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Strategies for Optimizing Heavy Metal Staining in Freeze-substituted Resin Embedded Plants Samples for Electron and X-ray Microscopy

Published online by Cambridge University Press:  30 July 2020

Kirk Czymmek ,
Keith Duncan ,
Ningning Zhang  and
Ru Zhang
Kirk Czymmek
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, Missouri, United States
Keith Duncan
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, Missouri, United States
Ningning Zhang
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, Missouri, United States
Ru Zhang
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, Missouri, United States

Abstract

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Type
Advances in Imaging Approaches for Plant Biology
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 142 - 143
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Copyright
Copyright © Microscopy Society of America 2020

References

Walther, P. and Ziegler, A., “Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water,” J. Microsc., vol. 208, no. 1, pp. 3–10, 2002.10.1046/j.1365-2818.2002.01064.xCrossRefGoogle ScholarPubMed
Johnson, E., Seiradake, E., Jones, E. Y., Davis, I., Grünewald, K., and Kaufmann, R., “Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins,” Sci. Rep., vol. 5, pp. 1–8, 2015.10.1038/srep09583CrossRefGoogle ScholarPubMed
Mcdonald, K. L. and Webb, R. I., “Freeze substitution in 3 hours or less,” J. Microsc., vol. 243, no. 3, pp. 227–233, 2011.10.1111/j.1365-2818.2011.03526.xCrossRefGoogle ScholarPubMed
Deerinck, T. J., Shone, T. M., Bushong, E. A., Ramachandra, R., Peltier, S. T., and Ellisman, M. H., “High-performance serial block-face SEM of nonconductive biological samples enabled by focal gas injection-based charge compensation,” J. Microsc., vol. 270, no. 2, pp. 142–149, 2018.10.1111/jmi.12667CrossRefGoogle ScholarPubMed
Bushong, E. A. et al. , “X-ray Microscopy as an Approach to Increasing Accuracy and Efficiency of Serial Block-face Imaging for Correlated Light and Electron Microscopy of Biological Specimens,” Microsc. Microanal., vol. 21, no. 1, pp. 231–238, 2015.10.1017/S1431927614013579CrossRefGoogle ScholarPubMed
Kittelmann, M., Hawes, C., and Hughes, L., “Serial block face scanning electron microscopy and the reconstruction of plant cell membrane systems,” J. Microsc., vol. 263, no. 2, pp. 200–211, 2016.10.1111/jmi.12424CrossRefGoogle ScholarPubMed
Jeiter, J., Staedler, Y. M., Schönenberger, J., Weigend, M., and Luebert, F., “Gynoecium and fruit development in Heliotropium sect. Heliothamnus (heliotropiaceae),” Int. J. Plant Sci., vol. 179, no. 4, pp. 275–286, 2018.10.1086/696219CrossRefGoogle Scholar
Czymmek, K., Sawant, A., Goodman, K., Pennington, J., Pedersen, P., Hoon, M., and Ortegui, M.S.. “Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy,” in Springer Protocols, Plant Endosomes, Ortegui, M. S., Ed. Springer US, 2020, p. In Press.Google Scholar
Hua, Y., Laserstein, P., and Helmstaedter, M., “Large-volume en-bloc staining for electron microscopy-based connectomics,” Nat. Commun., vol. 6, pp. 1–7, 2015.10.1038/ncomms8923CrossRefGoogle ScholarPubMed