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Staining of Mitochondria with Cy5-Labeled Oligonucleotides for Long-Term Microscopy Studies

Published online by Cambridge University Press:  09 May 2011

Steffen Lorenz
Affiliation:
Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
Stephanie Tomcin
Affiliation:
Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
Volker Mailänder*
Affiliation:
Max-Planck-Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany University Medicine of the Johannes Gutenberg University, III. Medical Clinic (Hematology, Oncology and Pulmonology), Langenbeckstraße 1, 55131 Mainz, Germany
*
Corresponding author. E-mail: [email protected]
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Abstract

Labeling of organelles for microscopy is achieved generally by specific dyes that either accumulate in a cellular compartment such as cyanine dyes in mitochondria or are only fluorescent under specific conditions such as the low pH in the lysosome. Here we demonstrate that Cy5—a fluorescent molecule that does not enter cells by itself—can be loaded into cells by attaching a short oligonucleotide. This very inexpensive labeling procedure can be done in the presence of serum. Therefore, very sensitive cell types should also be amenable to this procedure, and longer observations can be achieved compared to other commercially available dyes as the labeling reagent does not need to be washed out. This also points to the pitfall of using fluorescently labeled oligonucleotides for live cell imaging where the oligonucleotide is supposed to detect a specific target sequence in its subcellular distribution.

Type
Biological Applications
Copyright
Copyright © Microscopy Society of America 2011

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References

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