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Spectral Imaging of Chromogenic Dyes in Cytological Specimens

Published online by Cambridge University Press:  02 July 2020

Merryn Macville ,
Ernst-Jan Speel ,
Dirk Soenksen ,
Ton Hopman  and
ThomasRied
Merryn Macville
Affiliation:
National Human Genome Research Institute, NIH, Bethesda, MD20895
Ernst-Jan Speel
Affiliation:
Molecular Cell Biology, University of Maastricht, The Netherlands
Dirk Soenksen
Affiliation:
Applied Spectral Imaging Inc., Carlsbad, CA92009
Ton Hopman
Affiliation:
Molecular Cell Biology, University of Maastricht, The Netherlands
ThomasRied
Affiliation:
National Human Genome Research Institute, NIH, Bethesda, MD20895
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Extract

Recently, spectral imaging has been successfully applied to 24-color fluorescence in situ hybridization (FISH), and it has evolved into a new powerful method for the analysis of structural and numerical chromosomal aberrations on metaphase spreads (spectral karyotyping) For cytological specimens it is often impossible to use fluorescence microscopy because of (fixation-induced) auto-fluorescence or prior cytological staining. Also, re-examination of archived fluorescent specimens is hampered by fading. However, the need for multi-parameter cytochemical analysis remains when rare or unique material is to be studied in research or clinical diagnosis. Multi-color bright-field microscopy using enzyme precipitates has become feasible for in situ hybridization as well as immunohistochemical detection. Conventional transmission microscopy optics have allowed up to three targets to be detected simultaneously. In parallel to 24-color FISH, we explored the potential of spectral imaging to increase the number of parameters to be analyzed in bright-field microscopy.

Type
Cytochemistry, Histochemistry, Immunocytochemistry, and in Situ Hybridization
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 143 - 144
Copyright
Copyright © Microscopy Society of America 1997

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References

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