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Scanning Electron Microscopy Reveals Two Distinct Classes of Erythroblastic Island Isolated from Adult Mammalian Bone Marrow

Published online by Cambridge University Press:  22 February 2016

Jia Hao Yeo ,
Bronwyn M. McAllan  and
Stuart T. Fraser Open the ORCID record for Stuart T. Fraser [Opens in a new window]
Jia Hao Yeo
Affiliation:
Discipline of Anatomy & Histology, School of Medical Sciences, Bosch Institute, University of Sydney, Camperdown, NSW 2050, Australia
Bronwyn M. McAllan
Affiliation:
Discipline of Physiology, School of Medical Sciences, Bosch Institute, University of Sydney, Camperdown, NSW 2050, Australia
Stuart T. Fraser*
Affiliation:
Discipline of Anatomy & Histology, School of Medical Sciences, Bosch Institute, University of Sydney, Camperdown, NSW 2050, Australia Discipline of Physiology, School of Medical Sciences, Bosch Institute, University of Sydney, Camperdown, NSW 2050, Australia
*
*Corresponding author. [email protected]
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Abstract

Erythroblastic islands are multicellular clusters in which a central macrophage supports the development and maturation of red blood cell (erythroid) progenitors. These clusters play crucial roles in the pathogenesis observed in animal models of hematological disorders. The precise structure and function of erythroblastic islands is poorly understood. Here, we have combined scanning electron microscopy and immuno-gold labeling of surface proteins to develop a better understanding of the ultrastructure of these multicellular clusters. The erythroid-specific surface antigen Ter-119 and the transferrin receptor CD71 exhibited distinct patterns of protein sorting during erythroid cell maturation as detected by immuno-gold labeling. During electron microscopy analysis we observed two distinct classes of erythroblastic islands. The islands varied in size and morphology, and the number and type of erythroid cells interacting with the central macrophage. Assessment of femoral marrow isolated from a cavid rodent species (guinea pig, Cavis porcellus) and a marsupial carnivore species (fat-tailed dunnarts, Sminthopsis crassicaudata) showed that while the morphology of the central macrophage varied, two different types of erythroblastic islands were consistently identifiable. Our findings suggest that these two classes of erythroblastic islands are conserved in mammalian evolution and may play distinct roles in red blood cell production.

Keywords

erythroblastic islandsimmuno-gold labelingscanning electron microscopyerythropoiesismarsupials
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 2 , April 2016 , pp. 368 - 378
Copyright
© Microscopy Society of America 2016 

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